摘要
世界性害虫小菜蛾Plutella xylostella(L.)田间种群已对阿维菌素药剂产生了高抗药性。本研究以小菜蛾对阿维菌素的抗性品系、敏感品系和回交群体组建作图家系,再利用AFLP技术构建小菜蛾对阿维菌素抗性基因的连锁图谱。用10组AFLP引物组合对未经药剂处理的小菜蛾成虫DNA选择性扩增,共获得1044条可区分的DNA条带,271条带具有多态性,χ2检验表明只有123个位点符合1∶1(P=0.05),其中的112个构成28个连锁群,其总长度为1222.7cM;用10组AFLP引物对经药剂处理后存活的小菜蛾成虫DNA扩增,所得的DNA条带中有54个条带符合分离比1∶1,经Mapmaker3.0对多态性位点分析,只有E1M4-15、E1M1-4和E1M4-2标记位点位于同一连锁群,3个AFLP标记与抗性基因的遗传距离分别为0cM,8.3cM和13.1cM。比较分析得出,抗性基因所在的连锁群是第5连锁群。结果显示AFLP连锁图谱对小菜蛾对阿维菌素的抗性监测具有很好的应用潜力。
The diamondback moth, Plutella xylostella （L.） （Lepidoptera： Plutellidae）, is an important insect pest in cruciferous vegetables and has developed high resistance to avermectin all over the world. In this study, AFLP was used to construct a linkage map of resistance to avermectin in diamondback moth. The mapping families consisted of the susceptible strain, the abamectin-resistant strain and their backcross progeny. A total of 1 044 DNA bands and 271 polymorphic loci were amplified using 10 combinations of AFLP primers. Chisquare （x^2） analysis indicated that only 123 bands fit the segregation ratio of 1：1 （P = 0.05） in these polymorphic loci. Twenty-eight linkage groups were constructed with 112 bands, covering about 1 222.7 cM of the genomics of the diamondback moth. Three markers of ELM4-15, EIMI-4, and ELM4-2, linked with abamectin-resistant gene were situated on the same linkage group and their distance apart from resistant gene was 0 cM, 8.3 cM, and 13.1 cM, respectively. Comparison with the constructed linkage map showed that the gene was located on the fifth linkage group. These AFLP markers associated with resistance to abamectin show a strong potential of application in monitoring diamondback moth resistance to abamectin.
出处
《昆虫学报》
CAS
CSCD
北大核心
2007年第5期474-480,共7页
Acta Entomologica Sinica
基金
广东省自然科学基金项目(032021)
