柑桔爆皮虫卵巢发育动态

Ovarian development of Agrilus auriventris Saunders (Coleoptera:Buprestidae)

本研究利用室内饲养成虫,对柑桔爆皮虫的蛹及正常取食和交配、正常取食但未交配以及正常交配但未取食三种处理的雌成虫的卵巢发育进行了系统观察。结果显示:该虫具有1对卵巢,每侧有5根卵巢管。前两种处理的雌虫卵巢管均能正常发育,成虫寿命在30天左右;没有取食的雌虫寿命只有7天左右,在其卵巢管的生长区和成熟区均无卵形成。根据卵巢的形状、卵的产生过程、卵巢萼内有无卵粒以及卵黄沉积情况等将卵巢发育程度分为6个级别,即发育初期(0级)、卵黄沉积前期(Ⅰ级)、卵黄沉积期(Ⅱ级)、成熟待产期(Ⅲ级)、产卵盛期(Ⅳ级)和产卵末期(Ⅴ级)。每头雌虫最高怀卵量在140粒左右,根据雌虫怀卵量变化趋势,推测正常取食和交配的柑桔爆皮虫雌虫在出孔后10天左右开始产卵,产卵历期可达22天左右。据此提出该虫卵巢管发育到成熟待产期(Ⅲ级)之前(即羽化出孔后10天之内)为出孔成虫的防治适期。

Ovarian development of Agrilus auriventris Saunders was studied by dissecting the pupae, mated or unmated female adults fed with food, and mated females without any food. The results showed that the ovary of this pest has two ovaries, each composed of five ovarioles. Ovaries developed normally in the first two treatments of female adults fed with food, which had the longevity of about 30 days. While the mated female adults without any food had the longevity of only about 10 days, during which no eggs formed either in growth zone or maturation zone of ovarioles. Ovarian development was divided into 6 stages based on the morphological characteristics of ovarian development, the formation of eggs, eggs in the lateral oviducts and vitellogenesis： stage 0 （no oocyte stage）, stage Ⅰ （no yolk stage）, stage Ⅱ （yolk deposition stage）, stage Ⅲ （egg maturation stage）, stage Ⅳ （peak egg deposition stage）, and stage Ⅴ （egg depletion stage）. In mated adults with foods, mature eggs formed in 11 days after emergence, and the ovipositon duration could last as long as 22 days, with the highest fecundity of about 140 eggs per female. It is so proposed that to achieve better control effects, measuress should be taken to control the adults of A. auriventris before the stage Ⅲ of ovarian development （within 10 days after adult emergence）.