二氧化碳气腹对胃癌MKN-45细胞侵袭能力的影响

Effects of CO2 pneumoperitoneum on invasion and metastasis of MKN-45 gastric cancer cells

目的探讨CO2气腹对人胃癌细胞在裸鼠体内侵袭能力的影响。方法建立体外CO2气腹模型，实验组胃癌细胞暴露于CO2气腹2h（压力分别设为9、12、15mmHg，1mmHg=0．133kPa）。对照组胃癌细胞普通培养，然后注入裸鼠腹腔（2×10^6个细胞／只），每组10只。4周后每组取5只处死，记录腹腔及各脏器成瘤情况，剩余裸鼠观察生存时间。结果各组裸鼠腹腔种植成瘤率、不同脏器成瘤率差异均无统计学意义（P〉0．05）。腹腔内成瘤数：对照组（26．40±5．59）个，9mmHg组（25．40±5．27）个，12mmHg组（25．00±5．43）个，15mmHg组（22．40±5．37）个，各组间比较差异无统计学意义（P〉0．05）。裸鼠生存分析各组间差异无统计学意义（P〉0．05）。结论常用CO2气腹压力不会促使胃癌细胞裸鼠腹腔侵袭转移，不缩短被接种裸鼠的生存时间。

Objective To investigate the effects of CO2 pneumoperitoneum on the invasion and metastasis of MKN-45 gastric cancer cells in nude mice. Methods Forty nude mice were equally divided into 4 groups, i. e. 9 mm Hg group, 12 mm Hg group, 15 mm Hg group and control group. For the experimental groups, the MKN-45 gastric carcinoma cells were exposed to CO2 pneumoperitoneum of different pressures （9, 12 and 15 mm Hg） for 2 hours after the establishment of CO2 pneumoperitoneum model in vitro ; for the control group, the MKN-45 gastric cancer cells were exposed to 5% CO2 under 37℃. All the MKN-45 gastric carcinoma cells were injected into abdominal cavity of nude mice （2 ×10^6 cells/ mouse）. Five mice of each group were sacrificed 4 weeks later to record the number of tumor nodules in abdominal cavity and rate of viscera metastasis. The remaining mice were kept to observe the survival rate. Results There was no statistical significance of tumor metastasis in abdominal cavity and viscera among all the groups. The number of tumor nodules in control group, 9 mm Hg group, 12 mm Hg group and 15 mm Hg group was 26.40±5.59, 25.40 ±5.27, 25.00±5.43, 22.40±5.37, respectively, with no statistical difference among all the groups （ P 〉 0.05）. Conclusions The CO2 pneumoperitoneum would not increase the invasion and metastasis of MKN-45 gastric cancer cells in abdominal cavity of nude mice, and would not shorten the survival time of the inoculated mice.