摘要
建立了SPE净化和HPLC法测定土壤中常用除草剂咪唑乙烟酸的残留。土壤样品用0．1mol／L的醋酸铵与氨水缓冲液（pH10）超声波提取，提取液经酸化后用200mg／3mL C18 SPE柱净化，浓缩，乙腈定容后，供反相高效液相色谱检测，外标法定量。添加回收率为77．2％～94．9％，咪唑乙烟酸的最小检出量为5．2×10^-5μg，经计算可知，咪唑乙烟酸在土壤中的最小检出质量分数为0．2μg／kg。该方法大大减少了有机溶剂的使用，方便快速，结果准确可靠，适合一般实验室操作。
A new method for determining residues of the herbicide imazethapyr in soil was developed by means of reversed-phase high performance liquid chromatography （RP-HPLC）. The samples in soil were extracted with 0.1 mol/L NH4Ac/NH3·H2O（pH=10） water solution by ultrasonic wave, then acided by 1 mol/L hydrochloric acid solution and cleaned up by 200 mg/3mL C 18 SPE cartridge. The quantification of the herbicide residue was established by RP-HPLC. The results showed that the average recoveries for fortified soil samples （0.01-1 mg/kg） were 77.2 to 94.9%. The minimum detectable concentration of imazethapyr was 0.2 μg/kg in soil. The method reduces the application of organic solvent obviously and is simple, fast, and inexpensive with accurate and reliable for the determination of imazethapyr in soil for general laboratory.
出处
《农药》
CAS
北大核心
2007年第12期843-845,共3页
Agrochemicals
