染料木素分子印迹聚合物的合成与性能研究

Synthesis of molecularly imprinted polymers and their recognition study for genistein

目的研究染料木素分子印迹聚合物的合成及吸附性能。方法采用热聚合法,以染料木素为模板分子,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,在甲醇和N,N-二甲基甲酰胺(φ1∶φ2=9∶1)混合溶剂中制备分子印迹聚合物,并对聚合物进行表征;通过Scatchard方程考察了聚合物的结合特性及对其他结构相似的化合物的选择性吸附能力,研究聚合物的吸附动力学特征。结果聚合物通过氢键形成两类结合位点,并且对染料木素有比较明显的吸附特性。结论染料木素分子印迹聚合物有明显的选择性。

Aim To synthesize the molecular imprinted polymer for genistein and study the selective of the MIP.Methods Molecular imprinted polymers were prepared from acrylamide as functional monomers and ethylene glycoldimethacrylate as the cross-linker in methanol and N,N-dimethylformide（φ1∶φ2=9∶1）using genistein as the template molecular and 2,2＇-azobis-isobutyronitrile as the initiation agent.The binding characteristics of the template were evaluated by Scatchard analysis.The selective adsorption of MIP to different target molecular was studied.Results Our results indicated that there were two classes of recognized sites in the polymers and the MIP showed specific affinity toward genistein.Conclusion The MIPs will be used in the solid extraction and chromatrography separation.