四逆散、逍遥散与四君子汤对慢性应激肝郁模型大鼠行为学影响的研究

Effect of Powder Sini, Xiaoyao and Decoction of Sijunzi on behavior of stressed rats with chronic stagnation of liver-qi

[目的]探讨慢性应激大鼠模型的中医证候性质。[方法]SD雄性大鼠40只,用随机区组设计的方法分为对照组、模型组、四逆散组、逍遥散组和四君子汤组,观察大鼠体质量、血清皮质酮含量、1%蔗糖水摄取率和强迫游泳中不动时间。[结果]与对照组比较,模型组大鼠血清皮质酮含量升高(P<0.01),1%蔗糖水摄取率降低(P<0.05),强迫游泳中不动时间延长(P<0.01);与模型组比较,四逆散组、逍遥散组的血清皮质酮含量降低(P<0.05),1%蔗糖水摄取率升高(P<0.01),强迫游泳中不动时间缩短(P<0.05);各组大鼠体质量增加未见明显差异(P>0.05)。[结论]慢性应激大鼠模型属肝郁证与脾虚无关。

[Objective] To study the effects of Powder Sini, Xiaoyao and Decoction of Sijunzi on stagnation of liver-qi in rats with chronic unpredictable mild stress（CUMS） and explore the TCM syndrome characteristic of chronic stress rats. [Methods] Male Sprague-Dawley rats were exposed to an unpredictable sequence of mild stressors for 21 days and treated with Sini Powder, Xiaoyao Powder and Decoction of Sijunzi. Body weight, forced swimming test and fluid consumption were investigated during the whole procedure. Serum corticosterone was measured by ELISA. [Results] Rats exposed to CUMS showed decreased sucrose preference and increased time in forced swimming test, in- creased content of serum corticosterone（P〈0.01）. Regulating Liver and Spleen, Xiaoyao Powder could prevent theses changes （P〈0.01）, but Decoction of Sijunzi had no effects（P〉0.05）. [Conclusions] The model investigated in this chronic stressed rats belongs to liver stagnation syndrome and does not relate to spleen deficiency.