对二氯苯对小鼠肝肾抗氧化功能的影响

Effects of 1,4-DCB on lipid peroxidation and antioxidants in mice

目的:评价对二氯苯(p-DCB)亚急性染毒对小鼠的氧化性应激效应。方法:选用36只小鼠,雌雄各半,随机分成3组,每组12只,分别给予0(溶剂对照),450,900 mg/kg,每天灌胃1次,连续染毒1周。以丙二醛(MDA)作为脂质过氧化的产物指标;超氧化物歧化酶(SOD)代表抗氧化酶、还原性谷胱甘肽(GSH)代表非酶性抗氧化物综合评价氧化应激效应。结果:染毒后各组间体质量无显著性差别;染毒组肾体系数显著高于溶剂对照组(P<0.05)。小鼠肝脏及肾脏中SOD水平无显著性变化;高剂量组肾脏中GSH水平均低于对照组和低剂量组,与低剂量组比有显著性差别(P<0.05);染毒组肝脏和肾脏中MDA水平随着染毒剂量的增加逐渐升高,高剂量组MDA水平明显高于对照组和低剂量组。结论:p-DCB能降低小鼠组织的抗氧化功能,氧化性损伤可能是其重要的毒作用机制。

Objective： To evaluate the oxidative stress induced by subacute exposure to 1.4-dichloro- benzene（p-DCB） in mice. Methods： Thirty-six Kunming mice randomly divided into 3 groups, 0（ solvent control） ,450,900 mg p-DCB/kg b.w. p-DCB was administrated via garage, once a day for continuous 7 days. After One week, the level of GSH, SOD, MDA in the liver and kidney of mice were determined, Resuits： There is no significant difference on body weight among all treatment groups and control group, The kidney/body weight ratios of all treatment groups were increased significantly compared with control group. The activity of SOD in liver and kidney of all treatment groups were not significant compared with control group. The level of GSH in mice kidney in high dosage group decreased significantly compared with that of low dose groups. However there were no significance of the level of GSH in liver of three treatment groups ; compared with control and low dose groups, the level of MDA in mice liver and kidney in high dose group were increased significantly and with dose-dependently. Conclusion： Induction of oxidative stress may play a key role in the toxic action of p-DCB.