Cu(phen)_2^(2+)与6-巯基鸟嘌呤的相互作用

The Interaction among Cu(phen)_2^(2+),6-Mercaptopurine and DNA

目的为新抗癌药物的开发提供一定的理论研究依据。方法在Tris-NaC l(pH=7.2)缓冲溶液中,应用电化学实验、电子吸收光谱分析等技术研究了Cu(phen)22+(phen=1,10-邻菲咯啉)与6-巯基鸟嘌呤(6-TG)的相互作用。结果Cu(phen)22+与6-TG发生了明显的相互作用。一定量的6-TG存在时,Cu(phen)22+在铂电极上呈现的还原峰峰电位明显负移,峰电流明显减小;且Cu(phen)22+电子吸收光谱的最大吸收峰发生了明显的减色效应和红移现象。结论Cu(phen)22+与含巯基的嘌呤类物质存在较强的相互作用。

Objectiye： To provide the theretical basis for developing new andcancer drug. Methods ：The interaction of Cu （phen） 2^2＋ （ phen = 1,10 - phenanthroline） with 6 - Thiolguanine （ 6 - TG ） in Tris - NaC1 buffer solution （ pH = 7.2 ） was investigated by electrochemistry, electron absorption spectroscopy technique. Results：The results showed the interaction of Cu（phen） 2^2＋ with 6 -TG existed on the platinum disk electrode. However, peak potentials shifted negatively, peak currents decreased significantly in the presence of 6 - TG. At the same time, the maximal absorption peak from absorption spectra showed a red - shift and the intensity was weakened. Conclusion： The results indicate that the interaction of Cu （phen）2^2＋ and purine containing - SH group is strong as that of 6 -TG.