单侧咀嚼大鼠三叉神经节内降钙素基因相关肽的表达研究

Changes of Expressions of calcitonin gene-related peptide in Trigeminal Ganglia For Unilateral Chew in Rats

目的观察磨除单侧后牙造成偏侧咀嚼的大鼠三叉神经节内降钙素基因相关肽(calcitonin gene-relatedpeptide,CGRP)的表达情况,进一步探讨颞颌关节病的发病机制。方法Wistar雄性大鼠30只,随机分为6组,包括3个实验组及相应的对照组,每组5只。实验组动物磨除右侧上、下颌磨牙,人为造成单侧咀嚼。双侧三叉神经节(trigeminal ganglia,TG)切片行CGRP免疫组织化学反应(SABC法)。光镜观察拍片,并用Image Pro Plus 5.1图像分析软件分别进行测定。结果与对照组对照。结果每一实验组咀嚼侧和非咀嚼侧TG内CGRP免疫阳性神经元百分比与各自对照组比较显著降低(P<0.01,P<0.05),其非咀嚼侧明显低于咀嚼侧(P<0.01,P<0.05)。结论三叉神经节内CGRP参与了单侧咀嚼引起的颞颌关节病的病理过程,且两侧TG内CGRP释放量不同。

Objective：TO study the expressions of calcitonin generelated peptide（CGRP） in trigeminal ganglia in rats with unilateral Chew,and to discuss the mechanism of temporomandibular disorders.Methods：30 Wistar male rats were randomly divided into 6 groups,including 3 experimental groups and 3 control groups,5 rats in each group.The molar of right maxillary and mandibular of rats in experimental groups were ground to the gingival level without occlusal contact.The section of trigeminal ganglia underwent the immunohistological study to evaluate the expressions of CGRP by using SABC method.Light microscope and microscoic photo analytic software were used.Results： The percentage of CGRP positive neurons in TG in 3 experimental groups were significantly decreased compared with control group（P〈0.01,P〈0.05）,The decreased extent in non-chewing side was much higher than that in the chewing side（P〈0.01,P〈0.05）.Conclusion： The CGRP might participate in the histopathologic mechanism of temporomandibular disorders.The releas amount of CGRP in TG was different between chewing side and nonchewing under the condition of unilateral mastication.