葛花中总黄酮含量测定

Determination of Content of Flavonoids in Flos Puerariae

目的：建立用可见分光光度法测定葛花中总黄酮含量的方法．方法：乙醚脱脂，乙醇提取总黄酮．采用NaNO2，Al（NO2）2，NaOH显色，可见分光光度法测定总黄酮．结果：以芦丁为对照品作校正曲线，C=71．103A-0．810，r=0．9998，在12．32-61．60μg／ml范围内线性关系良好．葛花中总黄酮含量为0．75％．结论：方法简便、快速、灵敏、准确，可作为葛花中总黄酮的含量测定方法．

Objective： To establish a method for determination the content of flavonoids by visible spectrophotometry in the Chinese herbal medicine of Flos Pueraiae. Methods： Flavonoids in Flos Puerariae was extracted with the solvent ethanol after Ether degreasing. The NaNO2-Al （NO3）3-NaOH spectrophotometry was used to determine flavonoids. Results： Rutin was taken as the standard to make the standard curve： C=71. 103A-0.81, r=0. 9998, the linear range was 12. 32-61.60μg · ml^-1. The contents of flavoniods in Flos Pueraiae was 0.75%. Conclusion： This method was simple, accurate and suitable for the quality control of the flavonoids in Flos Pueraiae.