摘要
目的:建立用可见分光光度法测定葛花中总黄酮含量的方法.方法:乙醚脱脂,乙醇提取总黄酮.采用NaNO2,Al(NO2)2,NaOH显色,可见分光光度法测定总黄酮.结果:以芦丁为对照品作校正曲线,C=71.103A-0.810,r=0.9998,在12.32-61.60μg/ml范围内线性关系良好.葛花中总黄酮含量为0.75%.结论:方法简便、快速、灵敏、准确,可作为葛花中总黄酮的含量测定方法.
Objective: To establish a method for determination the content of flavonoids by visible spectrophotometry in the Chinese herbal medicine of Flos Pueraiae. Methods: Flavonoids in Flos Puerariae was extracted with the solvent ethanol after Ether degreasing. The NaNO2-Al (NO3)3-NaOH spectrophotometry was used to determine flavonoids. Results: Rutin was taken as the standard to make the standard curve: C=71. 103A-0.81, r=0. 9998, the linear range was 12. 32-61.60μg · ml^-1. The contents of flavoniods in Flos Pueraiae was 0.75%. Conclusion: This method was simple, accurate and suitable for the quality control of the flavonoids in Flos Pueraiae.
出处
《河北北方学院学报(自然科学版)》
2007年第6期33-34,38,共3页
Journal of Hebei North University:Natural Science Edition
基金
河北省中药局基金资助项目(0369)