不同储存时段血小板膜凋亡蛋白表达的变化

Expression of apoptosis protein in stored apheresis platelets

目的：分析不同储存时段血小板膜磷脂酰丝氨酸（PS）的表达变化，评价储存期间血小板的质量状况。方法：以凋亡受体Fas（CD95）体外诱导血小板凋亡，建立流式细胞术检测血小板PS的实验方法。测定不同储存时段30份血小板PS的静止表达率，同时检测经CD95刺激的血小板PS的诱导表达率。结果：在0、1、5、7和8d储存时间，静止血小板膜PS表达持续升高，表达率分别为2．03％±0．91％、3．26％±1．86％、8．98％±4．60％、26．68％±21．28％和38．85％±26．43％，各组间比较差异有显著性（P〈0．05～P〈0．001）。在CD95诱导作用下，各储存时间组血小板膜PS诱导表达率明显高于新鲜血小板0d组，组间比较差异有显著性（P〈0．01）。储存7d静止血小板PS表达率与CD95诱导0d组的诱导表达率比较（26．68％±21．28％和25．29％±18．90％）差异无显著性（P〉0．05）。结论：血小板膜凋亡蛋白PS的表达随储存时间延长而显著增加。新鲜血小板有抗凋亡诱导作用。血小板储存至7d，仍可能保持正常功能。

Objective To analyze the change of phosphotidyl serine （PS） in apheresis platelets （pits） during storage process, and to evaluate the quality of the plts during the storage period. Methods The apoptosis ligand Fas （CD95） was used to induce pits as apoptosis model, and by which the method to detect pits PS by flow cytometry （FCM） was established. Subsequently the resting PS expressive rate and the induced PS expressive rate of 30 apheresis pits, which induced by CD95, were determined using the FCM method established in storage process.Results At the 1st, 5th, 7th, 8th day of storage process, the PS expression of pits kept on increasing. The PS expressive rate of pits were 2.03% ±0.91%, 3.26± 1.86%, 8.98%± 4.60%, 26.68%±21.28%, and 38.85% ± 26.43 %, respectively, and there were significant differences among groups respectively （P〈 0.05）. Under induced-operation of CD95, the PS induced-expressive rate of every storage group was obviously higher than that of 0 day group （P〈0.01）. The PS expression rate of phs had no difference between 7-day storage group and 0-day induced group by CD95 （P〉0.05）. Conclusion The PS expression of apheresis plts increase significantly with the prolongation of storage time; Fresh plts has the ability of anti apoptosis; Apheresis plts could keep normal function until storage 7 d.