海洛因对大鼠肝功能血浆酶学的影响

Effect of heroin on enzyme in plasma of liver function in rats

目的:研究海洛因依赖大鼠肝功能的变化。方法:采用逐渐递增剂量法腹腔注射海洛因,建立海洛因依赖及戒断大鼠模型,50只建模成功Wistar大鼠随机分成对照组、给药3 d组、给药9 d组、戒断3 d组及戒断9 d组,检测各组大鼠血液生化指标天门冬氨酸氨基移换酶(AST)、丙氨酸氨基移换酶(ALT)及γ-谷氨酰基移换酶(GGT)。结果:与对照组比较,给药3 d组ALT的含量明显下降(P<0.05),给药9 d组ALT的含量下降更为显著(P<0.01),戒断3 d组ALT的含量上升显著(P<0.01);给药9 d组GGT的含量显著下降(P<0.01)。与给药9 d组相比,戒断3 d组AST的血浆含量显著升高(P<0.01);戒断3 d、9 d组的ALT血浆含量均显著升高(P<0.01);戒断3 d组血浆GGT含量明显升高(P<0.05),戒断9 d组血浆GGT含量升高更为显著(P<0.01)。结论:海洛因对肝功能的损害有延迟性及持续性,且不能在停药后立即消除。

Objective To investigate the changes of liver intraperitoneal injection with increasing doses to establish 50 Wistar rats were randomly divided into five groups group, 3 d withdrawal group and 9 d withdrawal group. function in heroin dependent rats. Methods Using rat models of heroin administration and withdrawal. control, 3 d administration group, 9 d administration The levels of aspartate aminotransferase （AST）, alanine aminotransferase （ALT） and γ-glutamyl transpeptidase （GGT） in plasma were tested. Results Compared with control group, the level of ALT in 3 d withdrawal group decreased significantly （P〈0. 01 ）, the level of ALT in 3 d administration group decreased significantly （P〈0. 05）, the level of AI.T in 9 d administration group decreased even significantly （P〈0. 01）, the level of ALT in 3 d withdrawal group increased apparently （P〈0. 01） ; and the level of GGT in 9 d administration group decreased apparently. Compared with 9 d administration group, the level of AST in 3 d withdrawal group increased apparently （P〈0. 01）, the levels of AI.T both in 3 d and 9 d withdrawal groups increased apparently （P〈0. 01）, the level of GGT in 3 d withdrawal group increased apparently （P〈 0.05）, and the level of GGT in 9 d withdrawal group increased even apparently （P〈0.01）. Conclusion The heroin has detention and durative damage to the liver function, and can＇ t be eliminated immediately after drug withdrawal.