Fe^(3+)-CMC对腹膜损伤模型大鼠腹膜损伤部位TGF-β及FGF表达的抑制作用

Inhibitory effect of Fe^(3+)-CMC on expressions of TGF-β and FGF in injured parts of postoperative peritoneal

目的:探讨Fe3+改性羧甲基纤维素(Fe3+-CMC)对术后腹膜损伤部位转化生长因子-β(TGF-β)及成纤维细胞生长因子(FGF)表达的抑制作用。方法:将60只大鼠制作成腹膜损伤模型,随机分为生理盐水对照组和Fe3+-CMC实验组;于术后1、3、5、7和14 d及2月时,各组随机取实验动物5只,麻醉后处死,剖腹探查,取损伤部位及与之邻接的粘连组织,应用免疫组织化学技术染色观察TGF-β、FGF的表达情况。结果:实验组腹膜损伤部位术后3、5、7和14 d时TGF-β、FGF阳性表达面积明显低于相同时间对照组,两组比较差异有显著性(P<0.05)。结论:Fe3+-CMC能够明显抑制术后腹膜损伤部位TGF-β、FGF表达,从而起到减少术后腹膜粘连的作用。

Objective To study the inhibitory effects of Fe3^＋ -CMC on the expressions of transforming growth factor β （TGF-β） and fibroblast growth factor （FGF） of injured parts of postoperative peritoneal. Methods Sixty Wistar rat models with abdominal adhesion were randomly divided into control group and expriment group. At the 1st, 3rd, 5th, 7th, 14th day and 2 months later, 5 rats were selected respectively in two groups and killed. The adhesion tissues were gained and the expressions of TGF-β and FGF were observed using immunohistochemistry technique. Results The expressions of TGF-β and FGF in expriment group at the 3rd, 5th, 7th and 14th day after operation were lower than those in control group at the same time （P〈0.05）. Conclusion Fe^3＋ CMC can inhibit the expressions of TGF-β and FGF of injured parts of postoperative peritoneal, then decrease the postoperation adhesion.