玉米花丝饮品对高脂血症鼠的降血脂作用

Action of corn filament beverage on falling blood fat in hyperlipemia rats and mice

目的:观察玉米花丝饮品(CFB)对高脂血症鼠的降血脂作用。方法:60只大鼠随机分为空白对照组、四氧嘧啶模型组、康立舒胶囊0.70 g.kg-1组及CFB 7.00、3.50和1.75 mL.kg-13个剂量组(n=10),每天给药1次,连续21 d;于给药第7、14、21天,测血清总胆固醇(TCHO)和甘油三酯(TG)含量,取肝脏测丙二醛(MDA)含量。取小鼠60只分组方法同上,每天给药1次,连续14 d;于第14天,测血清TCHO和TG含量,取肝脏测MDA含量。另取小鼠72只随机分为空白对照组、蛋黄模型组、排毒清脂胶囊0.4 g.kg-1组及CFB 10.0、5.0和2.5 mL.kg-13个剂量组(n=12),每天给药1次,连续10 d,末次给药前16 h除空白对照组外,所有各组小鼠每只均腹腔注射75%蛋黄生理盐水溶液0.5 mL,并且开始饥饿;末次给药后1 h测定血清TCHO及TG含量,取肝脏测MDA含量。结果:与模型组比较,CFB 7.00 mL.kg-1组大鼠血清TCHO和TG含量有明显降低趋势(P<0.001),CFB各剂量组大鼠肝组织MDA含量明显降低(P<0.001,P<0.001,P<0.05),并呈现出一定的量效关系。与四氧嘧啶模型组比较,CFB 10.0 mL.kg-1组小鼠血清TCHO和TG含量有明显降低趋势(P<0.01),CFB各剂量组小鼠肝脏组织MDA水平明显降低(P<0.001,P<0.001,P<0.05),并呈现出一定的量效关系。与蛋黄性模型组比较,CFB 10.0 mL.kg-1组小鼠血清TCHO含量有明显降低趋势(P<0.01),CFB 10.0和5.0 mL.kg-1组小鼠血清TG含量有明显降低趋势(P<0.01,P<0.05),CFB 10.0和5.0 mL.kg-1组小鼠肝脏组织MDA水平明显降低(P<0.001,P<0.01)。在降低TCHO和TG含量及小鼠肝脏MDA水平方面CFB 7.0 mL.kg-1组优于康立舒胶囊组,CFB10.0 mL.kg-1组优于排毒清脂胶囊组。结论:CFB对高脂血症鼠有明显的降血脂作用。

Objective To observe the action of lowering blood fat of corn filament beverage （CFB） on hyperlipemia rats and mice. Methods Sixty rats were randomly divided into control group, alloxan model group, kanglishu capsule 0.70 g · kg^-1 group, CFB 7.00, 3.50, 1.75 mL· kg^-1 groups （n=10） . Rats in each group were exhibited once a day for 21 d, on the seventh day, fourteenth day, twenty-first day, the contents of serum total cholesterol （TCHO） and triglyceride （TG） were determined, and liver malonaldehyde （MDA） level was measured. Another 60 mice were divided into 6 groups with the method mentioned above, mice in each group were exhibited once a day for 14 d, on the fourteenth day, the serum contents of TCHO and TG were determined, and liver MDA level was determined. Another 72 mice were randomly divided into control group, egg yolk model group, Expellant and Cleanning Fat Capsule 0.4 g · kg ^-1group, CFB 10.0, 5.0, 2.5 mL· kg^-1 groups （n=12）. Micein each group were exhibited once a day for 10 d. 16 h before the last administration, except control group, the mice in other groups were injected with 75% yolk physiological salt solution 0.5mL through the abdominal cavity, and began to starve, 1 h after the last administration, the contents of serum TCHO and TG were determined, and liver MDA level was measured. Results Compared with alloxan model group, TCHO and TG in hyperlipemia rats of CFB 7.00 mL · kg^-1 all reduced obviously （P〈0. 001）, the MDA content of each dose all reduced obviously （P〈0.001, P〈0.001, P〈0.01）, and showed a certain relation between quantity and effect. Compared with alloxan model mice, TCHO and TG in hyperlipemia mice of CFB 10.0 mL · kg ^-1 all reduced obviously （P〈0.01）, the MDA content of each dose all reduced obviously （P〈0. 001, P〈0.001, P〈0.01）, and showed a certain relation between quantity and effect. Compared with egg yolk model mice, TCHO in hyperlipemia mice of CFB 10.0 mL· kg^-1 all reduced obviously （P〈0.01）, TCHO in hyperlipemia mice of CFB 10.0, 5.0 mL · kg^-1 all reduced obviously （P〈0.01, P〈0.05）, the MDA contents of CFB 10.0, 5.0 mL· kg^-1 reduced obviously （P〈 0. 001, P〈0.01 ） . CFB 7.0 mL· kg^-1 was superior to Kanglishu Capsule in reducing TCHO, TG and MDA, CFB 10.0 mL · kg^-1 was superior to Expellant and Cleanning Fat Capsule. Conclusion CFB has the function of lowering blood fat on hyperlipemia rats and mice.