DcR3 mRNA在白血病细胞中的表达及意义

Expression of DcR3 mRNA in leukemia cells and its significance

目的:探讨DcR3 mRNA在急性白血病(AL)细胞中的表达及其临床意义。方法:选择AL患者46例,其中急性髓系白血病(AML)28例,急性淋巴细胞白血病(ALL)18例,27例非恶性血液病患者作为对照组,采用RT-PCR方法检测骨髓单个核细胞(BMNC)中DcR3 mRNA的表达水平,并分析其与临床参数的关联性。结果:AL组DcR3 mRNA表达阳性率(67.4%)及表达水平(0.56±0.09)高于对照组(40.7%,0.39±0.19)(P<0.05);AML组DcR3 mRNA表达阳性率(71.4%)及表达水平(0.56±0.09)与ALL组(61.1%,0.53±0.08)比较差异无显著性(P>0.05);AL患者DcR3 mRNA的阳性表达率在白细胞计数≥30×109.L-1时升高。结论:DcR3基因可能通过抑制白血病细胞凋亡途径参与白血病发病过程。

Objective To investigate the expression of DcR3 mRNA in cells of acute leukemia （AL） and its clinical significance. Methods The DcR3 mRNA of bone marrow mononuclear cells （BMNC） in the patients with AL was detected by RT-PCR. There were 28 AML cases, and 18 ALL cases. 27 cases of non-malignant hematopathy were used as control group. The expression level of DcR3 mRNA in BMNC was detected with RT-PCR. Results The positive rate of DcR3 mRNA was 67.4% and gene expression level was 0.56±0. 09 in AL group, while they were 40. 7% and 0. 39±0.19 in control group, there were significant differences （P〈0.05）. The positive rate of DcR3 mRNA was 71.4% and the gene expression level was 0.56 ± 0.09 in AML group, while they were 61.1% and 0.53±0.08 in ALL group, there was no significant difference （P〉0.05）. The positive expression level of DeR3 mRNA increased when the white blood cell count was ≥30× 10^9· L^-1.Conclusion DcR3 gene might play a role by suppressing apoptosis in leukemogenesis.