玉米种皮固态发酵绿色木霉生产纤维素酶的研究

Production of Cellulase in Solid State Fermentation with Corn Seed Capsule by Trichoderma Viride

通过以玉米种皮为基质对绿色木霉J98-06的固态发酵研究,选育得到一株高产纤维素酶菌株,其最适培养条件为:玉米种皮与麦麸质量比3:7,硫酸铵1.4%,K2HPO4 0.2%(质量分数),加水量为1:1.2～1.4(质量比),发酵温度30℃,发酵时间4天,产酶活力高达3865 U/g(干曲)。

Trichoderma viride J98-06 that has very high cellulase activity was isolated in solid-state fermentation with corn seed capsule as the substrate. The study indicated that the optimal parameters of the culture were： the ratio of corn seed capsule and wheat bran was 3 ; 7, the ratio of dry raw material and water was 1 ： 1.2～1.4 （m/m）, （NH3）2SO4 1.4%, K2HPO4 0.2%. CMCase activity could reach to 3865 U/g after cultivation at 30℃ for 96 h.