hIGF-1工程细胞对糖尿病小鼠血糖的影响

EFFECTS OF HIGF-1 ENGINEERING CELLS ON BLOOD GLUCOSE IN DIABETES MELITUS MICE

目的:观察hIGF-1工程细胞移植对糖尿病模型小鼠的降血糖作用。方法:用高病毒滴度的病毒上清感染骨髓基质细胞,并植入糖尿病模型小鼠腹腔内,观察糖尿病模型小鼠血糖的变化。结果:稳定表达hIGF-1的骨髓基质细胞腹腔移植后,可见糖尿病小鼠的血糖明显下降(P<0.05)。结论:稳定表达hIGF- 1的骨髓基质细胞对糖尿病动物模型有降糖作用,说明IGF-1基因可作为糖尿病基因治疗的候选基因。

Objective：To investigate the effects of decreasing blood glucose that transplant hIGF-1 engi- neering cells to diabetic model mice.Methods：The marrow stromal cells were infected with high virus titer supernatant and transplanted into the diabetic model mice abdominal cavity.The changes of blood glucose were detected.Results：After the diabetic model mice were transplanted with bone marrow stromal cells that stably express hIGF-1 gene,the blood glucose decreased obviously （P 〈 0.05）.Conclusions：The marrow stromal cells that stably express hIGF-1 gene can obviously decrease blood glucose of diabetic model mice,which suggest that hIGF-1 gene can be used as the candidate gene for gene therapy of diabetes mellitus.