hIGF-1腺相关病毒质粒的构建、包装和滴度测定

CONSTRUCTION,PACKAGEAND TITER DETERMINATION OF PAAV/HIGF-1 PLASMID

目的:构建pAAV/biGF-1载体,并测定pAAV/hIGF-1病毒粒子包装盒病毒滴度。方法:将hIGF- 1基因克隆入pAAV腺病毒相关病毒载体,以HEK293包装细胞包装病毒颗粒,HT1080细胞检测病毒滴度。结果:获得插入方向正确的hIGF-1腺相关病毒载体,HEK293细胞包装得到了1012滴度的病毒上清。结论:成功构建了hIGF-1腺相关病毒载体,并且获得了较高滴度包装病毒颗粒。

Objective：To construct pAAV/hlGF-1 plasmid and determine viral titer.Methods：hIGF-1 cDNA fragment was cloned into pAAV vector. Viral particles were packaged with HEK293 cells and viral titer was determined with HT1080 cells.Results：pAAV/hIGF- 1 plasmid with right inserted direction was obtained. After packaged by HEK293 cells,the viral titer was up to 1012.Condusions：pAVW hIGF-1 plasmid was constructed successfully and higher viral titer was obtained.