摘要
目的:探讨检测血液中端粒酶活性(TA)和癌胚抗原信使核糖核酸(CEA mRNA)诊断消化系统恶性肿瘤血液转移的应用价值。方法:选择123例消化系统恶性肿瘤患者(肿瘤组,其中肿瘤转移患者64例、未发现转移的患者59例)和124例非肿瘤性消化道疾病患者(对照组)为对象,应用端粒末端重复序列扩增-杂交-酶联免疫检测技术检测TA、逆转录-套式-聚合酶链反应技术检测CEA mRNA。结栗:肿瘤组TANCEA mRNA阳性率明显高于对照组(P<0.01),肿瘤转移组TANCEA mRNA阳性率明显高于未转移组(P<0.01),TA和CEA mRNA诊断消化系统肿瘤血液转移的特异性、敏感性、阳性预测值、阴性预测值分别为88.1%和91.5%、79.7%和89.1%、87.90/0和91.9%、80.0%和88.5%。结论:检测血液标本中TA和CEA mRNA对诊断消化系统恶性肿瘤血液转移具有较高的应用价值。
Objective:To investigate the value of detecting telomerase activity (TA) and Carcinoembryonic antigen mRNA (CEA mRNA) for diagnosing hematogenous metastasis of digestive system malignant tumor. Methods: 123 digestive system malignant tumor patients (tumor group that includes 64 metastasis patients and 59 non-metasta- sis patients) and 124 non-tumor digestive system diseases patients (non-tumor group) were used in this study;TRAP- Hyb-ELISA and RT-NP-PCR were respectively used to detect TA and CEA mRNA.Results:The TA and CEA mRNA positive rate of tumor group were obviously higher than that of non-tumor group (P 〈 0.01) ;The TA and CEA mRNA positive rate of metastasis patients were significantly higher than that of non-metastasis patients (P 〈 0.01).The specificity, sensitivity, positive predictive value and negative predictive value of TA and CEA mRNA in diagnosing of hematogenous metastasis of digestive system cancer were 88.1% and 91.5%,79.7% and 89.1%,87.9% and 91.9%, 80.0% and 88.5% respectively.Conclusions:Decting TA and CEA mRNA in blood has higher applying value in diagnosing hematogenons metastasis of digestive system malignant tumor.
出处
《承德医学院学报》
2007年第4期361-363,共3页
Journal of Chengde Medical University