循环TA和CEA mRNA指标诊断消化系统恶性肿瘤血液转移

STUDY ON THE VALUE OF DETECTING TAAND CEA MRNAFOR DIAGNOSING HEMATOGENOUS METASTASIS OF DIGESTIVE SYSTEM MALIGNANTTUMOR

目的:探讨检测血液中端粒酶活性(TA)和癌胚抗原信使核糖核酸(CEA mRNA)诊断消化系统恶性肿瘤血液转移的应用价值。方法:选择123例消化系统恶性肿瘤患者(肿瘤组,其中肿瘤转移患者64例、未发现转移的患者59例)和124例非肿瘤性消化道疾病患者(对照组)为对象,应用端粒末端重复序列扩增-杂交-酶联免疫检测技术检测TA、逆转录-套式-聚合酶链反应技术检测CEA mRNA。结栗:肿瘤组TANCEA mRNA阳性率明显高于对照组(P<0.01),肿瘤转移组TANCEA mRNA阳性率明显高于未转移组(P<0.01),TA和CEA mRNA诊断消化系统肿瘤血液转移的特异性、敏感性、阳性预测值、阴性预测值分别为88.1%和91.5%、79.7%和89.1%、87.90/0和91.9%、80.0%和88.5%。结论:检测血液标本中TA和CEA mRNA对诊断消化系统恶性肿瘤血液转移具有较高的应用价值。

Objective：To investigate the value of detecting telomerase activity （TA） and Carcinoembryonic antigen mRNA （CEA mRNA） for diagnosing hematogenous metastasis of digestive system malignant tumor. Methods： 123 digestive system malignant tumor patients （tumor group that includes 64 metastasis patients and 59 non-metasta- sis patients） and 124 non-tumor digestive system diseases patients （non-tumor group） were used in this study;TRAP- Hyb-ELISA and RT-NP-PCR were respectively used to detect TA and CEA mRNA.Results：The TA and CEA mRNA positive rate of tumor group were obviously higher than that of non-tumor group （P 〈 0.01） ;The TA and CEA mRNA positive rate of metastasis patients were significantly higher than that of non-metastasis patients （P 〈 0.01）.The specificity, sensitivity, positive predictive value and negative predictive value of TA and CEA mRNA in diagnosing of hematogenous metastasis of digestive system cancer were 88.1% and 91.5%,79.7% and 89.1%,87.9% and 91.9%, 80.0% and 88.5% respectively.Conclusions：Decting TA and CEA mRNA in blood has higher applying value in diagnosing hematogenons metastasis of digestive system malignant tumor.