生理剂量雄激素在雄性大鼠实验性血栓形成中的调节作用

Effect of Physiological Concentration of Androgen on Experimental Thrombosis in Male Rats

目的研究生理剂量雄激素在实验性血栓形成中的作用,并进一步探讨雄激素对凝血与纤溶系统的调节。方法成年雄性Wistar大鼠去势模型与实验性血栓模型制备;血浆睾酮(testosterone,T)应用ADVIA Centaur Immunoassay System测定;而二氢睾酮(dihydrotestosterone,DHT)应用酶联免疫吸附测定(ELISA);凝血与纤溶参数采用STA-R血凝分析仪检测;血浆黏度应用SA-6000自动血液流变学检测仪测定。结果雄性大鼠去势后血浆睾酮与二氢睾酮明显降低(7.94±3.07 nmol/L vs 0.96±0.09 nmol/L;1.76±0.77 nmol/L vs 0.1±0.02 nmol/L,P均<0.05)。在40%三氯化铁(40%FeCl3)刺激下,去势大鼠腹主动脉血栓形成的面积与重量明显增加(1157.38±68.74μm2vs 969.43±22.66μm2;7.17±1.72 mg vs 5.17±1.17 mg,P均<0.05)。去势大鼠血浆凝血酶原活动度(PA%)显著升高(68.00±4.74 vs 53.00±1.81,P<0.05),而凝血酶原时间(PT)、活化的部分凝血酶时间(aPTT)、国际标准化比值(INR)显著降低(P均<0.05)。去势大鼠血浆纤维蛋白原(Fbg)、纤溶酶原活性(PLG:A)、D二聚体(D-dimer)、血浆黏度与假手术大鼠间无差别(P均>0.05)。结论生理浓度的雄激素介导对雄性大鼠凝血过程的调节,抑制实验性血栓的形成。

Objectives The aim of our study is to if experimental thrombosis is regulated by physiological doses of androgen via modulating coagulation and fibrinolytic processes. Methods Castrated models and experimental thrombosis models of male rats were prepared. Total plasma testosterone was measured using ADVIA Centaur Immunoassay System. Whereas the dihydrotestosterone （DHT） levels were measured using a DHT ELISA kit. s of blood coagulation and fibrinolytic system were assayed using STA- R Coagulation analyzers, and plasma viscosity was tested using SA-6000 automated hemorrheology tester. Results Total plasma testosterone anti DHT were significantly lower in castrated rats than in normal rats （7.94 ± 3.07 nmol/L vs 0.96 ± 0.09 nmol/L; 1.76 ± 0.77 nmol/L vs 0.1±0.02 nmol/L, （ all P 〈 0.05 ）. Castration caused significant increase in the thrombus area and weight in castrated rats as compared with control group （1157.38 ± 68.74 μm^2 vs 969.43 ±22.66 μm^2 ;7.17 ± 1.72 mg vs 5.17 ± 1.17 mg, all P 〈0.05 ）. Prothrombin activity （ PA% ） was higher, and PT , aPTT and INR were lower in castrated rats than in normal rats （ all P 〈 0.05）. There weren＇ t significant difference in FIB, D- dimer and plasma between in castrated rats and in normal rats （ all P 〉 0.05 ）. Conclusion Inhibition of experimental thrombosis by physiological doses of androgen is mediated via modulating coagulation processes in male rats.