用驯化的活性污泥强化修复氯酚污染土壤的试验

Bioaugmentative remediation of chlorophenol-contaminated soil with acclimated activated sludge

以接种驯化的活性污泥为生物强化手段，通过摇瓶反应模拟生物泥浆反应器的运行，研究了受氯酚污染土壤的修复特性。结果表明，接种驯化的活性污泥可以大大加快邻氯苯酚（2-CP）的降解速率，对2-CP初始污染浓度为500mg／kg干土的土壤，接种1％活性污泥（w：w）后反应11h降解率即可达到96．4％。最适的反应条件为：活性污泥接种量1％，水土比2：1，温度25℃，摇床转速200r／min。2-CP的降解符合表观一级动力学方程；且当初始浓度为50～500mg／kg干土时，2-CP降解速率常数随着初始浓度的增大而减小。

Studies were carried out on slurry-phase bioremediation of chlorophenol-contaminated soil in flask, adding acclimated activated sludge for bioaugmentation. The results showed that addition of acclimated activated sludge could greatly improve the degradation of 2-chlorophenol （2-CP）. After adding 1% （w： w, dry weight） activated sludge, 2-CP was degraded by 96.4% in 11 hours when its initial concentration was 500 mg/kg （dry weight）. The optimum parameters were as follows： the inoculation ratio of activated sludge to soil, 1% ; the ratio of water to soil, 2：1 ; temperature, 25 ℃ ; rotating speed, 200 r/min. The degradation of 2-CP can be described by the pseudo-first-order reaction model. When the initial concentration of 2-CP was 50 ～ 500 mg/kg （dry weight） , the higher the initial concentration, the lower the pseudo-first-order rate constant.