慢肾1号方对小鼠系膜增生性肾炎免疫分子等超微结构干预的对照性研究

Comparative study of interference of No1 therapy of chronic nephritis on microstructure of immune molecule in mice with MsPGN

目的:研究中药慢肾1号方对系膜增生性肾炎小鼠模型的自由基损伤及免疫病理学等超微结构的干预作用。方法:采用注射大肠杆菌内毒素(LPS)的方法建立小鼠慢性肾炎病理模型,观察小鼠一般情况,测定其24h尿蛋白定量、肾功能、血超氧化物歧化酶(SOD)、丙二醛(MDA)的变化结果。结果:治疗组小鼠一般情况明显好于模型对照组及中、西药对照组,24h尿蛋白显著少于模型对照组(P<0.01)和中药对照组(P<0.05),与正常组比较无显著差异,SOD活力较模型对照组明显提高(P<0.05),MDA含量则显著降低(P<0.01);治疗组与中药对照组和西药对照组比较亦有显著差异(P<0.05)。结论:慢肾1号方对系膜增生性肾炎有良好的治疗作用,其治疗慢性肾炎的机理可能与该方调节机体免疫功能、抑制炎症反应,提高抗氧化酶活性,减轻或阻断脂质过氧化链锁反应,减轻自由基损伤有关。

Objective ：To study interference of No1 therapy of chronic nephritis on free radical injure and microstructure of immune in mice with MsPGN. Methods：Model of mice with chronic nephritis by injection of LPS were established,and the general condition were observed. The changes in 24 h urine protein,kidney function, SOD and MDA were detected. Results： Compared with the model control group,Tradition Chinese treatment group and western medicine treatment group , the general condition of mice in the treatment group was superior than that of other groups,with 24 h urine protein obviously less than the model control group（P〈0.05） and Tradition Chinese treatment group（P〈0.01）;activity of SOD significantly enhanced（P〈0.05） ;content of MDA obviously reduced （P〈0.01）. There were also significant differences between Tradition Chinese treatment group and western medicine treatment group. Conclusion：No1 therapy of chronic nephritis has favorable therapeutic effect on MsPGN and the mechanism is related to the regulation of immune function, inhibition of inflammation reaction, increase of the activity of anti-oxidization enzyme, relief of free radical injure etc.