摘要
目的:观察华佗再造丸对急性出血性脑卒中大鼠中枢神经二次损伤的治疗作用,并探讨其可能作用机理。方法:自体血脑内注入造成大鼠急性出血性脑卒中模型,观察华佗再造丸对模型大鼠中枢神经二次损伤以及脑内纤溶酶原(PLG)和组织型纤溶酶原激活物(tPA)的影响,以及对血脑屏障及细胞超微结构的保护作用。结果:(1)大约70%模型大鼠于造模后4d～6d再次出现神经功能缺损体征,中枢神经二次损伤于造模后6d～8d达到高峰。(2)自用药后第4d起,华佗再造丸组大鼠神经功能缺损评分一直维持在(1.24±0.19)分左右,未出现明显中枢神经二次损伤现象。第7d华佗再造丸组大鼠神经功能缺损评分为(1.22±0.15)分,与模型组第7d比较,差异有显著性(P<0.05)。(3)模型组大鼠第3d中枢tPAmRNA特异扩增产物及第7d中枢PLGmRNA特异扩增产物均明显高于假手术组;华佗再造丸组tPAmRNA、PLGmRNA表达明显低于模型组。(4)华佗再造丸能减轻血脑屏障通透性和细胞超微结构损伤,对脑出血大鼠血脑屏障有保护作用。结论:华佗再造丸能有效治疗急性出血性脑卒中大鼠中枢神经二次损伤,这一作用与抑制急性出血性脑卒中大鼠中枢tPAmRNA及PLGmRNA表达有关。
Objective： To observe the effects of Huatuozaizao Pill on Acute Hemorrhagic Apoplexy rats whose central nerve were hurt for the second time. And the mechanisms were also investigated. Methods： Rats models were made by blood of itself injected in brains. Huatuozaizao Pill＇s were given for them. On the end, PLG and tPA in brain were examined. Meanwhile, Protect effects on blood--brain barrier and cell- ultrastructure were probed. Results ： （1） Neurologic impairment signs were emergenced in 70 % rats after 4-6 days. Its peak was in 6-8 days. （2） The score of neurologic impairments were 1.24±0.19 after treating 4 days. The second hurts were no emergence. But the score is 1.22±0.15 after 7 days. And there were significant difference compared with that of model group. The express of tPA,mRNA and PLG mRNA in treated group were lower than that of model group. Blood-brain barrier and cell-ultrastructure impairments were also relieved. Conclusions： Huatuozaizao Pill had good effects on Acute Hemorrhagic Apoplexy rats whose central nerve were hurt for the second time. Their mechanisms were related to the express of tPA mRNA and PLG mRNA.
出处
《世界中西医结合杂志》
2007年第8期442-446,共5页
World Journal of Integrated Traditional and Western Medicine
基金
上海市医学领军人才项目(No.BR97016)
