补阳还五汤对脊髓损伤后胶质瘢痕及GFAP表达的影响

Effect of Buyang Huanwu Decoction on Glial Scar and GFAP Expression after Spinal Cord Injury

目的:探讨补阳还五汤对脊髓损伤(SCI)后胶质瘢痕及胶质纤维酸性蛋白(GFAP)表达的影响。方法:成年雌性SD大鼠24只,随机分为假手术组、实验组和对照组,每组8只,实验组和对照组在C3～4右外侧索横断后分别以补阳还五汤和蒸馏水连续灌胃8w,取脊髓损伤部位,行苏木素染色和GFAP免疫组化染色,对胶质瘢痕的体积、GFAP阳性细胞相对面积和相对灰度值进行测定。结果:实验组胶质瘢痕的体积小于对照组(P<0.01);实验组GFAP阳性细胞相对面积和相对灰度值明显低于对照组(P<0.01)。结论:补阳还五汤能减少脊髓损伤后胶质瘢痕的体积,抑制星形胶质细胞的活化和增生。

Objective：To explore the effect of Buyang Huanwu Decoction （invigorating Yang for recuperation） on glial scar and GFAP expression after spinal cord injury. Methods. 24 adult female SD rats were randomized into three groups： the normal, the experimental and the control, with an average of 8 rats in each group. Upon lesions of their right-sided lateral funiculus at C3-4, the experimentals and the controls were given respectively the intragastric administration of Buyang Huanwu Decoction and distilled water for 8 weeks. Haematoxylin staining and immunohistochemical staining for GFAP were applied to the lesions to detect the glial scar volume, relative surface area and grey-scale value of GFAP-positive cells, Results. The experimental group was reported to have smaller glial scar volume than the control group （P〈0.01）. Relative surface area and grey-scale value of GFAP-positive cells in the experimental group were also lower than those in the control group （P〈0.01）, Conclusion： Buyang Huanwu Decoction has the efficacy of reducing glial scar formation after spinal cord injury, inhibiting astrocyte activation and proliferation.