高效诱导甜菜再生植株的研究

High Efficient Induction of Sugar Beet Plant Regeneration

研究了栽培甜菜（ＢｅｔａｖｕｌｇａｒｉｓＬ．）４倍体品系４０５叶柄外植体的离体培养，成功地建立了一套高频率诱导再生芽的程序。外植体取自生长在改良ＭＳ（ＭＳＢ）附加ＢＡ和ＮＡＡ或者单加ＢＡ的培养基中，经过３０ｄ以上预培养后的幼苗叶柄，在ＭＳ附加ＢＡ１０ｍｇ／Ｌ或ＮＡＡ０３ｍｇ／Ｌ，ＢＡ１０ｍｇ／Ｌ培养基上直接诱导再生芽，并发育成苗，诱导频率最高可达５１３％。在１／２ＭＳ（ＭＳ培养基大量元素减半）附加ＮＡＡ０５～１０ｍｇ／Ｌ的培养基上诱导生根。

A method for high frequency in vitro regeneration of adventitious shoots from petiole explants was established on a tetraploid breeding line 405 of Beta vulgaris L.Plant regeneration was obtained by direct bud formation from petioles.Explants were precultured in a medium containing MS inorganic salts supplemented with Gamborgs′ B5 vitamins,named MSB.Adventitious shoots were developed when petiole explants excised from 20～30 days old seedlings grown on MSB containing 0.3mg/L naphthalene acetic acid (NAA),1.0mg/L 6 benzylaminopurine (BA) and then were cultured on MS with 0.3mg/L NAA,1.0mg/L BA.Shoots rooted on half strength MS medium containing 0.5～1.0mg/L NAA.This procedure of the culture method may be very useful both for multiplication of sugarbeet and for introducing foreign genes into Beta vulgaris.