摘要
用绿色荧光蛋白基因作为筛选标记的新型克隆载体的构建李寿东齐义鹏胡建红林宏(武汉大学病毒研究所武汉430072)目前通常使用的质粒克隆载体,如pUC系列、pGEM系列和pBluescript等,都是以lacZ’作为筛选标记的插入失活型载体。lacZ’...
A novel cloning vector named pQLgreen was derived from pUC19 by insertion the ORF of gfp S65T gene into SmaI site of pUC19,under the control of LacZ promoter operator.Host bacteria transformed by pQLgreen may grow to blue colonies on Ap/IPTG LB plates.Green tag lost when the multiple restriction sites upstream the gfp S65T ORF was inserted by foreign DNA fragments.The colonies of host bacteria with recombinant plasmid showed colorless.So we incorporate a new colour selection for insertion,different from the blue and colourless selection system.It is economical because it does not require the use of costly X gal.
出处
《生物工程学报》
CAS
CSCD
北大核心
1997年第3期323-325,共3页
Chinese Journal of Biotechnology
基金
国家自然科学基金
国家九五攻关项目资助