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基于酶催化沉积放大的阻抗免疫传感器

An amplified immunosensor has been proposed based on precipitation of an insoluble product on functionalized electrode
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摘要 提出一种基于酶催化沉积底物和生物素——亲和素两步放大的超灵敏免疫检测方法.抗体通过单层蛋白A固定到金电极表面,预先处理好的生物素化抗体和辣根过氧化物酶(HRP)标记的亲和素形成的网状复合物作为免疫反应信号的放大探针.在有H2O2存在的情况下,通过辣根过氧化物酶(HRP)催化氧化DAB(3,3’—联苯二胺)在金电极上沉积一层不溶物,引起阻抗的显著增大.该传感器用于人IgG检测,检测下限为1.0×10-8g.采用法拉第阻抗和压电石英晶体微天平分别表征了该传感器的特性. An amplified immunoassay with improved sensitivity has been proposed based on two - step amplifieation procedures of enzyme - catalyzed precipitation and biotin - streptavidin binding system. Anti - IgG antibody is mobilized onto the surface of gold electrode modified with a thin layer of protein A. Biotin labeled anti - IgG antibody and HRP - streptavidin are premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction. HRP biocatalyzes DAB ( 3,3'- diaminobenzidine) in the presence of, resulting in an insoluble product on the electrode surface, to achieve an enhanced electron - transfer resistance on the electrode surface as well as a decreased frequency. The results show that this method can greatly improve the detection sensitivity of IgG in human serum with concentration as low as 1. 0 ×10^- 8 gmL^-1. We employe Faradaic impedance spectroscopy and quartz crystal microbalance (QCM) to follow the stepwise assembly of the systems and investigate immunosensing properties.
作者 黄晓梅
出处 《宁德师专学报(自然科学版)》 2007年第4期357-361,共5页 Journal of Ningde Teachers College(Natural Science)
关键词 生物传感器 酶催化沉积放大 生物素——亲和素 biosensors biocatalyzed precipitation biotin - streptavidin binding system
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  • 1杨晓达,常文保,慈云祥.免疫分析法进展[J].化学进展,1995,7(2):83-97. 被引量:12
  • 2高志贤.压电免疫生物传感器的研究进展[J].军事医学科学院院刊,1995,19(4):310-313. 被引量:4
  • 3楚霞,林朝晖,沈国励,俞汝勤.甲胎蛋白压电免疫传感器的研究[J].高等学校化学学报,1996,17(6):870-873. 被引量:15
  • 4陈誉华,宋今丹,李大为.核酸探针传感器的构建[J].生物化学与生物物理进展,1996,23(6):527-531. 被引量:8
  • 5高志贤,张超,陶桂全,黎高翔,冯冠平.压电免疫传感器抗体固化方法研究[J].中华微生物学和免疫学杂志,1996,16(3):191-195. 被引量:17
  • 6[2]Xing WL, Ma LR, Jiang ZH, et al. Portable fiber-optic immunosensor for detection of methsulfuron methyl[J]. Talanta, 2000, 52(5): 879-883.
  • 7[3]Naimushin AN, Soelberg SD, Nguyen DK, et al. Detection of Staphylococcus aureus enterotoxin B at femtomolar levels with a miniature integrated two-channel surface plasmon resonance (SPR) sensor[J]. Biosensors Bioelectron, 2002, 17(6-7): 573-584.
  • 8[4]Wong YY, Ng SP, Ng MH, et al. Immunosensor for the differentiation and detection of Salmonella species based on a quartz crystal microbalance[J]. Biosensors Bioelectron, 2002, 17(8): 676-684.
  • 9[5]Chaki NK, Vijayamohanan K. Self-assembled monolayers as a tunable platform for biosensor applications[J]. Biosensors Bioelectron, 2002, 17(1-2): 1-12.
  • 10[8]Sergeyeva TA, Lavrik NV, Rachkov AE, et al. An approach to conductometric immunosensor based on phthalocyanine thin film[J]. Biosensors Bioelectron, 1998, 13(3-4): 359-364.

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