内源性高甘油三酯血症患者体内存在氧化型低密度脂蛋白、极低密度脂蛋白和高密度脂蛋白

Oxidative Modification of Plasma Low Density Lipoprotein, Very Low Density Lipoprotein and High Density Lipoprotein in Endogenous Hypertriglyceridemia

为探讨内源性高甘油三酯血症患者体内低密度脂蛋白、极低密度脂蛋白及高密度脂蛋白是否发生氧化修饰，对25例内源性高甘油三酯血症患者与25例年龄性别相匹配的正常人的血糖、血脂、载脂蛋白及脂过氧化物进行了分析，并对其血浆低密度脂蛋白、极低密度脂蛋白及高密度脂蛋白的电泳迁移率、234nm光吸收和硫代巴比妥酸反应物质含量的改变进行了测定。结果表明，内源性高甘油三酯血症患者血浆甘油三酯、脂过氧化物和载脂蛋白B100、CⅢ、CⅢ及E显著升高（P＜0．01），高密度脂蛋白和载脂蛋白AⅠ显著降低（P＜0．01），低密度脂蛋白、极低密度脂蛋白和高密度脂蛋白电泳迁移率均较正常人相应脂蛋白的迁移率增加，234nm光吸收及硫代巴比妥酸反应物质含量均显著增加（P＜0．01）。说明内源性高甘油三酯血症患者不仅血浆低密度脂蛋白、而且极低密度脂蛋白和高密度脂蛋白均发生了氧化修饰。

Aim Oxidized low density lipoprotein (LDL), verylow density lipoprotein (VLDL ) and high densitylipoprotein(HDL) were occured in vivo in endogenoushypertriglyceridemia (HTG ) human.Methods Human plasma triglycerides (TG), totalcholesterol (TC), high density lipoprotein cholesterol(HDLC), apolipoproteins A l, AⅡ, B100, CⅢ, Eand lipoperoxide (LPO) levels in 25 endogenous HTGpatients whose plasma TG levels were>2. 26 mmol/Land TC<6. 21 mmol/L, and in 25 normal age-matchedpersons (TG < 2. 26 mmol/L, TC < 6. 21 mmol/L )were measwred. Blood glucose, TC, TG and HDLCwere performed by enzyme method. Plasmaapolipoprotein were determined by radial immunuodiffusion (RID) assay. The human plasma LDL, VLDLand HDL were isolated by the one step density gradientultra-centrification method. The oxidative modificationof LDL, VLDL and HDL was identified by agarose gelelectrophoresis, absorbance at 234 nm and fluorescenceof thiobarbituric acid reaction substance(TBARS).Results The results showed that plasma TG, LPOand apolipoprotein B100 levels in endogenous HTGgroup were significantly higher than that of the controlgroup (P<0. 01). Plasma HDLC and apolipoprotein AⅠ contents in endogenous HTG group were significantly lower than that of the control group (P< 0.01). The electrophoretic mobility of LDL,VLDL andHDL was increased and absorbance at 234 nm andTBARS of LDL, VLDL and HDL in endogenous HTGgroup were significantly higher than that of the controlgroup (P<0. 01).Couclusion The results suggest that not only LDLbut also VLDL and HDL were oxidatively modified invivo of endogenous HTG patients-