降钙素基因相关肽对大鼠主动脉内皮剥脱的保护作用

The Protective Role of Calcitonin Gene-Related Peptide on Balloon Denuded Injurying of Rat Aorta

为研究降钙素基因相关肽在防止动脉内膜损伤后平滑肌细胞增殖所致再狭窄中的作用机制，采用内皮剥脱、血管条灌流和放射免疫测定法测定了环磷一酸尿苷、降钙素基因相关肽和内皮素水平。结果发现，内皮剥脱后两周，血浆及主动脉组织降钙素基因相关肽含量增加（前者对照组为9．2±1．7，去内皮组为14．1±2．0ng／L；后者对照组为7．4±1．4，去内皮组为12．2±2．0ng／g）。应用外源性降钙素基因相关肽（25μg/Kg）可明显改善损伤血管对乙酰胆碱的内皮依赖性舒张反应；促进去内皮血管壁一氧化氮的合成；增加主动脉组织内cGMP的含量（对照组4．4±0．5，去内皮组2．0±0．5，降钙素基因相关肽组3．3±0．5ng/g；P＜0．01），减少内皮素的生成（P＜0．01），抑制损伤部位的内膜过度憎生（P＜0．01）．提示外源性降钙素基因相关肽可通过促进内皮的修复、抑制内膜的过度增殖而防止剥脱血管的再狭窄。

Aim This work investigated the protective role ofcalcitonin gene-related peptide (CGRP) on balloon de-nuded injury of rat aorta.Methods Per fusion of denuded arterial rings, de-termination of cGMP and endothelin (ET) with re-dioactive analysisResults 2 weeks after denudation, the contents ofplasma and aorta CGRP were increased. ExogenousCGRP (25 μg/kg, iv) could significantly improve therelaxation of denuded aorta to acetyl choline, inducenitric oxide (NO) synthesis (P<0. 01 ), and inhibitthe over proliferation of denuded intima (P<0. 01 ).Couclusion Exogenous CGRP could prevent therestenosis of balloon denided aorta through promtingthe repairment of denuded endothelium, inhibiting theover proliferation of injured intima.