碱性纤维母细胞生长因子对实验性家兔急性心肌梗塞的影响

The Effect of Basic Fibroblast Growth Factor on Acute Myocardial Infarction in Rabbits

为探讨碱性纤维母细胞生长因子对急性心肌梗塞的影响及临床应用前景，用冠状动脉结扎法制备了家兔急性心肌梗塞模型．在此模型上亿脉注射碱性纤维母细胞生长因子1．4μg/kg，共6次．结果发现能明显缩小心肌梗塞范围，梗塞心肌重量占心脏重量百分比分别为8．1％±2．7％和8．2％±2．1％，对照组为16．5％±3．2％（P＜0．05）；增加心输出量和心肌收缩力（±LVdq／dtmax显著增加）；增加梗塞区心肌ATP贮备（2．9±0．4和3．2±0．3μmol／g，对照组为2．3±0．3μmol／g，P＜0．01）．结果提示，碱性纤维母细胞生长因子具有促进梗塞心肌愈合和改善心功能的作用，且这一作用明显优于临床上常用的扩血管药硝酸甘油。

Aim To investigate the effect of basic fibroblastgrowth factor (bFGF) on acute myocardial infarction(AMI) in rabbits.Methods The hearts of heathy male Japan big-earwhite rabbits were exposed through a left thoracoto-my, and the left anterior descending coronary arterywas ligated with a silk. Animals were divided intocontrol group, intravenously infused normal saline (2ml/kg) nitroglycerin (0. 2 g/kg), bFGF 1. 4 pg/kgand 7. 0 μg/kg, respectively, on operation say and onthe 2nd, 4th, 6th, 8th, and 10th day afer operation.We measured the areas of infarct myocardium with te-trazolium chloride and cardiac function by using physi-ological polygraphy, and determined ATP contents inmyocardium.Results Intravenous infusion of bFGF (1. 4 μg/kgand 7. 0 μg/kg) decreased the infarct size significantly(8. 1%±2. 7% and 8. 2%±2. 1% vs control 16. 5%±3. 2%, P<0. 05), Treatment with bFGF alsoincreased the ATP content in infarct myocardium (2. 9±0. 4 and 3. 2±0. 3 vs control 2. 3±0. 3 μmol/g, P<0. 01) and improved the left ventricular. All the effectof bFGF were better than those of nitroglycerin(P<0. 05).Conclusion bFGF may play a role both in stimu-lating recovery of ischemic myocardium and in improv-ing cardiac function in AMI rabbits.