亮氨酸脑啡肽抑制血管平滑肌细胞增殖和c-fos基因表达

Inhibitory Effects of Leucine-Enkephalin on the Proliferation and c-fos Proto-oncogene and Expression of Vascular Smooth Muscle Cell

为探讨脑啡肽与去甲肾上腺素一起参与血管运动的局部调节机制，用MTT法、氚标胸腺嘧啶脱氧核苷掺入法和斑点杂交技术发现亮氨酸脑啡肽可抑制SD大鼠胸主动脉平滑肌细胞的增殖、DNA合成和c-fos基因的表达；阿片受体阻断剂纳洛酮无上述抑制作用，但可拮抗亮氨酸脑啡肽的上述抑制作用；上述作用还可被去甲肾上腺素所拮抗。结果提示，脑啡肽通过影响血管平滑肌细胞功能来参与血管运动的局部调节，这种调节可能是通过阿片受体介导的。

Aim To investigate the effects of leucine-enkepha-line(L-ENK) on the proliferation, the DNA synthesisand c-fos oncogene expression of vascular smooth mus-cle cell (VSMC).Methods Techniques of cell culture, MTTmethod, 3 H-thymidine incorporation and Dot blot.Results L-ENK (10-5 mol/L) could markedly in-hibit the proliferation, DNA synthesis and c-fos onco-gene expression of VSMC. Naloxone (10-6 mol/L),opioid receptor antagonist, had no effect on the prolif-eration, DNA synthesis and c-fos oncogene expressionof VSMC, but, naloxone could antagonized the in-hibitory action of L-ENK to the proliferation ofVSMC. The inhibitory action of L-ENK to the VSMCcould be antagonized by the noradrenaline (10-5mol/L),adrenoceptor activator.Conclusion L-ENK inhibit the proliferation, DNAsynthesis and c-fos oncogene expression of VSMC byactivation of opioid receptors.