谷氨酰胺增强氧化应激时肺微血管内皮细胞热休克蛋白70的表达

Glutamine enhances the expression of heat shock protein 70 in pulmonary microvascular endothelial cells under oxidative stress conditions

目的研究谷氨酰胺(Gln)对氧化应激状态下体外培养肺微血管内皮细胞(pulmonary microvascular endothelial cells,PMVEC)热休克蛋白(HSP)70表达的影响。方法采用体外培养Wistar大鼠PMVEC,分为阳性对照组(G组)、生理盐水组(PS组)、谷氨酰胺组(Gln组)、过氧化氢加生理盐水组(H2O2+PS组)和过氧化氢加谷氨酰胺组(H2O2+Gln组)。Western blotting检测PMVEC HSP70的表达情况,分别记录平均灰度值。结果与PS组(9.05±1.51)相比,Gln组(40.28±10.19)和H2O2+PS组(43.89±10.32)PMVEC HSP70表达分别增高4.5倍和5倍(P<0.01);与H2O2+PS组相比,H2O2+Gln组(65.59±14.53)HSP70表达进一步增高,约为PS组的7倍,接近G组(70.56±12.23)表达水平。结论谷氨酰胺可增强氧化应激状态下体外培养肺微血管内皮细胞HSP70表达。

Objective To investigate the effects of glutamine on the expression of heat shock protein （HSP） 70 in pulmonary micro-vascular endothelial cells （PMVECs） under oxidative stress conditions. Methods PMVECs from Wistar rats were divided into 5 groups： the positive control group （G group）, the physiologic saline group （PS group）, the glutamine group（Gin group）, the H2O2 ＋ PS group and the H2O2 ＋ Gin group. Western blotting was used to investigate the expression of HSP70. Results Compared with the PS group, the expression of HSP70 significantly increased in the Gin group（9.05± 1.51 vs 40.28± 10.19） and the H2O2 ＋ PS group（9.05 ± 1.51 vs 43.89 ± 10.32）. The expression of HSP70 was higher in the H2O2 ＋ Gin group than in the H2O2 ＋ PS group（65.59 ± 14.53 vs 43.89 ± 10.32）. Conclusion Glutamine enhances the expression of HSP70 in pulmonary micro-vascular endothelial cells under oxidative stress conditions.