Metallothionein expression induced by nickel accumulation in the midgut of Spodoptera litura Fabricius larvae exposed to nickel

Metallothionein expression induced by nickel accumulation in the midgut of Spodoptera litura Fabri- cius larvae was investigated by exposing S. litura larvae to the artificial diets amended with different doses of nickel for 3 generations. Nickel accumulations in the midgut of 6th instar larvae were detected by Inductively Coupled Plasma-Atomic Emission Spectrometer (ICP-AES), and the induced metal- lothionein expression in the midgut of 5th and 6th instar larvae in 120 h were quantified using cad- mium-hemoglobin total saturation methods. The accumulated nickel in the midgut of the 6th instar larvae within a generation increased with the increase of the nickel doses in the treated diets and showed significant dose-dependence with the nickel doses in diets. Metallothioneins induced by nickel accumulated in the larval midgut also increased with nickel doses in diets and with the increasing stressing time in a generation. Results also suggested that the metallothionein expression was differ- ent with the time of S. litura larvae stressed by nickel.

Metallothionein expression induced by nickel accumulation in the midgut of Spodoptera litura Fabricius larvae was investigated by exposing S. litura larvae to the artificial diets amended with different doses of nickel for 3 generations. Nickel accumulations in the midgut of 6th instsr larvae were detected by Inductively Coupled Plasma-Atomic Emission Spectrometer （ICP-AES）, and the induced metsl-Iothionein expression in the midgut of 5th and 6th instsr larvae in 120 h were quantified using cadmium-hemoglobin total saturation methods. The accumulated nickel in the midgut of the 6th instsr larvae within a generation increased with the increase of the nickel doses in the treated diets and showed significant dose-dependence with the nickel doses in diets. Metsllothioneins induced by nickel accumulated in the larval midgut also increased with nickel doses in diets and with the increasing stressing time in a generation. Results also suggested that the metsllothionein expression was different with the time of S. litura larvae stressed by nickel.