糖皮质激素受体-α在慢性鼻及鼻窦炎黏膜中的表达和意义

Expression and significance of glucocorticoid receptor-α in mucosa of chronic rhinosinusitis

目的检测慢性鼻及鼻窦炎(chronic rhinosinusitis,CRS)患者鼻黏膜中糖皮质激素受体-α(glucocorticoid receptor-α,GR-α)的表达,探讨其在CRS发病中的作用。方法以14例激素敏感型和激素不敏感型CRS患者中鼻甲、中鼻道侧黏膜作为研究对象,应用免疫组化SP法和实时荧光定量逆转录聚合酶链反应检测GR-α的表达,以7例正常鼻黏膜作为对照组,并对表达结果进行统计学分析。结果GR-α在CRS和对照组中均有阳性表达,在激素敏感型CRS中表达强度高于激素不敏感型CRS和对照组(P均<0.05);激素不敏感型CRS与对照组表达无明显差异(P>0.05)。结论GR-α表达失调可能是CRS黏膜对糖皮质激素敏感性不同的原因之一。

OBJECTIVE To detect the expression of glucocorticoid receptor-α（GR-α）in mucosa of chronic rhinosinusitis （CRS） patients and to investigate the role of GR-α in CRS pathogenesis. METHODS Immunohistochemistry and fluorescent real time quantitative PCR （FQ-RT-PCR） were performed on middle turbinate mucosa in 7 steroid sensitive and 7 insensitive CRS patients and 7 controls. RESULTS Positive expressions of GR-α was detected in CRS groups and controls. Increased protein and RNA expression of GR-α in steroid sensitive CRS group was found compared with that in insensitive group and control group （P〈 0.05）. The difference of expression was not found between insensitive CRS patients and controls （P〉0.05）. CONCLUSION The variable response of CRS to steroid treatment may be the result of differences in the expression of GR-α.