预聚合法制备聚丙烯酰胺毛细管凝胶柱

A New Technique of Pre-polymerization for Preparation of Polyacry-amide Gel Filled Capillaries for Capillary Gel Electrophoresis

提出了一种温和条件下制备交联聚丙烯酸胺毛细管凝胶电泳柱的新方法──预聚合法．讨论了制备过程中的问题，获得了凝胶组成、毛细管内径及长度等不同的交联聚丙烯酸胺毛细管凝胶电泳柱．采用激光回射干涉检测技术。

A novel method 'pre-polymerization' for production of cross-linked polyacrylamide gel filled-capillaries was studied in detail. Many factors of this method which contribute to the ratio of success for producing the gel-filled capillaries were discussed. i. e.,characters and pureness of Acr and Bis, use level of TeMED and AP, effect of pH value and other characters of buffer, time of polymerization, diameter and length of capillaries, and conditions of preservation and so on. This method was demonstrated to be applicable to the production of gel-filled capillaries with wide varieties of gel composition, capillary diameters and capillary lengh. Over 9O% ratio of success for producing the gel-filled capillaries was obtained with this method. Retroreflective laser beams interference phase-shift detection was used for on-column detection of proteins in cross-linked polyacrylamide gel capillary electrophoresis (CPAGCE) firstly. For ovalbumin, 4. 2 ×10-7 mol/L(S/N= 2) of detection limit was measured and 1. 66 ×1O5 theoreticaｌplates was observed on the home-made CE system. It should prove attractive for a number of applications in separation of biological macromolecule samples with the gel-filled capillaries produced by the method of pre-polymerizatlon.