丹酚酸B对小鼠脑缺血不同时间脑能量代谢及脑水肿的作用

Effect of salvianolic acid B on brain energy metabolism and hydrocephalus of cerebral ischemia in mice at different time

复制脑缺血小鼠模型,测定缺血6,12,18,24及30 m in脑组织的能量负荷(EC)、磷酸肌酸(PCr)、乳酸(Lac)的含量、Na+-K+-ATPase活性、脑指数和脑含水量,观察丹酚酸B(Sal B)对小鼠脑缺血不同时间的脑能量代谢的影响,并探讨其对脑水肿的作用。结果发现,脑缺血6 m in后,EC值、PCr含量、Na+-K+-ATPase活性开始下降,而Lac含量、脑指数和脑含水量逐渐升高。应用丹酚酸B(22.5 mg.kg-1)可改善小鼠各缺血时间点的病理生理变化,特别是在脑缺血30 m in时段,丹酚酸B可显著升高EC值和PCr含量(P<0.01);增强Na+-K+-ATPase活性(P<0.05);同时降低Lac含量(P<0.01)、脑指数(P<0.01)和脑含水量(P<0.05)。结果提示,Sal B可能通过改善小鼠急性脑缺血能量代谢从而起到减轻脑水肿的作用,该研究为临床应用丹酚酸B治疗脑缺血提供了科学的依据。

Mice pathological model of acute cerebral ischemia was established.In order to observe the effect of salvianolic acid B（Sal B） on brain energy metabolism and hydrocephalus in the brain of mice at different ischemic times,the energy charge（EC）,content of phosphocreatine（PCr）,level of lactic acid（Lac）,activity of Na^＋-K^＋-ATPase,brain index and water content of brain were measured at 6,12,18,24,and 30 min,separately after ligating bilateral common carotid arteries in mice.NIH mice were randomly divided into sham-operated group（sham）,cerebral ischemia group（ischemia）,Sal B-treated group（Sal B） and nimodipine-collated group（Nim）.At 6 min after cerebral ischemia,EC,content of PCr and activity of Na^＋-K^＋-ATPase began to decrease,while level of Lac,brain index and water content of brain increased gradually.However,Sal B（22.5 mg·kg^-1） improved pathophysiological changes at different ischemic times.Especially at 30 min after cerebral ischemia in Sal B group,EC（P〈0.01）,content of PCr（P〈0.01） and activity of Na^＋-K^＋-ATPase（P〈0.05） increased significantly.Meanwhile,level of Lac（P〈0.01）,brain index（P〈0.01） and water content of brain（P〈0.05） were lower obviously than those of cerebral ischemia group.Sal B could alleviate hydrocephalus by the improvement of energy metabolism in mice with acute cerebral ischemia,that provides scientific evidence that Sal B can be used for the clinical application of ischemic diseases.