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肿瘤坏死因子α诱导人乳腺癌MCF7/hGFP-Bax稳定表达细胞凋亡对Bax转移定位的影响 被引量：1

Effect of Bax translocation on TNFα induced apoptosis of human breast cancer MCF7 cells stably expressed hGFP-Bax
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摘要 建立人乳腺癌MCF7细胞hGFP-Bax稳定表达细胞系,观察肿瘤坏死因子α(TNFα)诱导的细胞凋亡对Bax自细胞浆转移定位至线粒体的影响。荧光显微镜观察凋亡细胞Bax从细胞浆至线粒体的转移和对细胞核染色体断裂的影响,并观察和测定TNFα诱导细胞凋亡的量效和时效关系。以免疫荧光法观察GFP-Bax从细胞浆转移至线粒体和定位以及细胞色素c(Cyt-c)从线粒体的释出,MTT法测定TNFα的细胞毒作用,TMRE测定观察对细胞线粒体功能的影响。结果显示TNFα诱导的MCF7/GFP-Bax细胞凋亡与Bax自细胞浆转移定位于线粒体密切相关。 Established human breast cancer MCF7 cell line stably expressed GFP-Bax,which was used to investigate Bax translocation from cytosol into mitochondria induced by TNFα.Bax translocation from cytosol into mitochondria and DNA fragmentation were investigated under fluorescence microscope.The viability of cells treated with TNFα was measured by MTT method.Immunofluoresence method was used to investigate Bax translocation to mitochondria and Cyt-c release from mitochondria.The Dose-effect and time-course relationship were also observed and calculated by the percentage of GFP-Bax punctate cells.The TMRE assay was used to investigate the function of mitochondria.The results showed that Bax translocation from cytosol into mitochondria was associated with apoptosis induced by TNFα in hGFP-Bax-tagged MCF7 stable cell line.
作者 侯琦
机构地区 中国医学科学院中国协和医科大学药物研究所
出处 《药学学报》 CAS CSCD 北大核心 2007年第12期1254-1258,共5页 Acta Pharmaceutica Sinica
关键词 肿瘤坏死因子Α 细胞凋亡 BAX TNFα apoptosis Bax
分类号 R963 [医药卫生—微生物与生化药学]
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参考文献12

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同被引文献11

  • 1Depraetere V, Golstein P. Fas and other cell death signaling pathways [ J ]. Semin Immunol, 1997,9:93 - 107.
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  • 5Hou Q, Hsu YT. Bax translocation from cytosol to mitochondria in cardiac cells during apoptosis : development of a GFP-Bax-stable H9c2 cell line for apoptosis analysis [ J]. Am J Physiol Heart Circ Physiol, 2005,289 : H477 - H487.
  • 6Hou Q, Cymbalyuk E, Hsu SC, et al. Apoptosis modulatory activities of transiently expressed Bcl-2: role in cytochrome c release and Bax regulation [ J ]. Apoptosis, 2003,8:617 - 629.
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  • 9Smilli SS, Hsu YT, Sanders KM, et al. Bax translocation to mitochondria subsequent to a rapid loss of mitochondrial membrane potential [ J ]. Cell Death Differ, 2001,8:909 - 920.
  • 10Kim B, Ryu S, Song B. JNK- and p38 kinase-mediated phosphorylation of Bax leads to its activation and motochondria translocation and to apoptosis of human hepatoma HepG2 cells [ J ]. J Biol Chem, 2006,281 : 21256 - 21265.

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药学学报
2007年 第12期

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