银杏叶提取物预防大鼠急性肝损害机制的研究

Protective effect of Ginkgo biloba extract on acute hepatic injury induced by carbon tetrachloride in rats.

目的观察银杏叶提取物对大鼠急性肝损害的预防作用及其机制,寻找临床适用的检测指标。方法四氯化碳诱发大鼠急性肝损害,同时使用银杏叶提取物进行预防,使用生化检测仪检测丙氨酸氨基转移酶和天冬氨酸转氨酶的含量改变;使用比色法检测超氧化物歧化酶和丙二醛的含量变化;采用酶联免疫吸附测定方法检测肿瘤坏死因子α和转化生长因子β1的含量变化。结果银杏叶提取物有效降低了丙氨酸氨基转移酶和天冬氨酸转氨酶的含量;并使超氧化物歧化酶的含量显著升高,与之对应丙二醛的含量显著降低;肿瘤坏死因子α和转化生长因子β1的含量明显降低。结论银杏叶提取物可以有效预防大鼠急性肝损害,其机制是抑制了氧化应激反应和调节了细胞因子网络。上述检测指标和研究方法适合在临床推广。

Objeαive To investigate the proteαive effeα of Ginkgo biloba extraα （GbE） on acute hepatic injury induced by carbon tetrachloride （ CC14 ） in rats. Methods Acute hepatic injury was indicated by the raise of levels of Aspartate Aminotransferase （AST） and Alanine Aminotransferase （ALT）. At the same time, expressions of superoxide dismutase（SOD） , malondialdehyde（ MDA）, tumor necrosis faαor α （TNF- α） and transforming growth faαor β1 （TGF - β1 ） were also deteαed by colorimetry and ELISA. Results There was a remarkable improvement of serum ALT, AST in the GbE - treated group. SOD was decreased while MDA was increased correspondingly. Under the effeα of GbE, both serum levels of TNF - αand TGF - β1 were increased in rats treated simply with CC14. But under the effeα of GbE, the two signal molecules were de- creased to a significant low level, although still higher than those of controls. Conclusions GbE can partially proteα rat liver from the acute injury induced by CC14. The mechanism may lie in its effeα of inhibiting oxidative stress caused by liver injury and expressions of signal molecules such as TNF - α and TGF - β1- GbE may thus be of potential help as a medicament for alleviation of hepatic injury.