雷公藤修饰的树突状细胞延长大鼠小肠移植物存活时间的研究

Study on prolonging survival of intestinal allograft by administration of Tripterygium Wilfordii modified dendritic cells.

目的研究雷公藤修饰的供体树突状细胞(DC)延长大鼠小肠移植物存活时间的作用。方法体外培养供体大鼠DC,并与雷公藤共培养48 h。实验动物分3组,受体于手术前分别预处理后进行小肠移植。Ⅰ组:注射生理盐水;Ⅱ组:注射供体未修饰的DC;Ⅲ组:注射雷公藤修饰的供体DC。观察受体存活时间,移植小肠病理学检查,用酶联免疫吸附试验(ELISA)法检测受体血清白介素-2(IL-2)、干扰素-γ(INF-γ)水平。结果III组受体动物存活时间明显长于Ⅰ、Ⅱ两组(P<0.05),组Ⅲ移植小肠炎性细胞浸润、黏膜结构破坏程度、血清IL-2、INF-γ水平均明显低于Ⅰ、Ⅱ组(P<0.01)。结论术前输注雷公藤修饰的DC,可抑制大鼠小肠移植后的排斥反应,延长小肠移植物的存活时间。

Objective To investigate the effects of Tripterygium wilfordii modified dendritic cells （DCs） on prolonging the survival time of intestinal allograft in rats. Methods DCs were collected from progenitor cells in bone marrow of Wistar rats, and they were cultured with GM - CSF and IL-4 in vitro. Tripterygium glycosides （Tw） were applied for 48h on day 7. The antigen presenting by DC was evaluated by mixed lymphocyte responses. Seven days before transplantation of small intestine from Wistar donors, normal saline （ group Ⅰ）, non - modified DCs （ group Ⅱ ） and Tripterygium wilfordii modified DCs （ group Ⅲ ） were infused intravenously into SD recipient rats, respectively. Small bowel transplantation was performed and the survival time of recipients was observed. Their histological examinations were evaluated, and serum IL - 2 and INF - γ levels were assayed by ELISA. Results Tripterygium wilfordii modified DC exhibited weak activity in stimulating the proliferation of allogenic T cells. The survival time of recipients in group Ⅲ was significantly longer than that in group Ⅰand group Ⅱ The degree of infiltration of inflamma- tory cells and structural destruction in intestinal mucosa was significantly milder than that of group I. The concentration of serum IL - 2 was much lower in group ~I in comparison with that of group Ⅰ and group Ⅱ.The concentration of serum INF - γ was obviously lower in group Ⅲ in comparison with that of group Ⅰ and groupⅡ. Conclusion The modification of DCs with Tripterygium wilfordii can prolong the survival time of intestinal allograft after transplantation.