幽门螺旋杆菌多重感染同临床转归的关系

The association between multi-strain infection of Helicobacter Pylori and its clinical outcomes

目的探讨vacA不同等位基因组合(即不同的s与m的组合)及幽门螺旋杆菌多重菌株感染同临床转归的关系。方法对胃镜下确诊为慢性胃炎和消化性溃疡的患者进行胃黏膜H.pylori培养并提取DNA分析vacA基因的表型并同时确定患者是否存在多重菌株感染。结果从87位患者胃黏膜中培养出幽门螺旋杆菌并提取DNA,vacA等位基因组合以s1 c和m2 a分布为主,未发现s1b和s2;消化性溃疡和非消化性溃疡患者不同vacA等位基因的分布率无显著性差异;36例患者的菌株显示具有一种以上的s或m等位基因型,推断为多重菌株感染,混合菌株感染率为41.4%。消化性溃疡和非消化性溃疡患者多重菌株的感染率无显著性差异。结论我国幽门螺旋杆菌多重菌株感染率高,未发现多重菌株感染同消化性溃疡相关,亦未发现vacA等位基因同消化性溃疡发病相关。

Objective To investigate the relationship between Helicobacter pylori multi-strain infection and clinical outcomes. Methods Helicobacter pylori were cultured from gastric biopsy of patients with gastritis or ulcer in stomach or deodedum. DNA abstracted from bacteria was analyzed to determine the alleles＇type of vacA. Results slc,ml and m2 were dominant among the 87 patients; Multi-strain infection rate was 41.4% , much higher than those in western countries. There was no significance difference of multi-strain infection rate between patients with peptic ulcer disease and patients with non peptic ulcer disease. Conclusions Multi-strain infection was not associated with the clinical outcome of helicobacter pylori infection.