PTTG、c-Myc及Ki67在胃癌中的表达及相关性研究

Expression and relationship of PTTG,c-Myc and Ki67 in gastric carcinoma

目的检测PTTG、c-Myc及Ki67在胃癌、慢性萎缩性胃炎及正常胃黏膜中的表达,研究三者在胃癌表达中的相关性,探讨三者对胃癌发生、发展、转移、预后的影响及机制。方法采用免疫组织化学SP法检测PTTG、c-Myc及Ki67在60例胃癌组织、20例慢性萎缩性胃炎和10例正常胃黏膜中的表达。结果PTTG、c-Myc及Ki67表达在正常胃黏膜、慢性萎缩性胃炎及胃癌组织中差异均有显著性(P<0.05);按胃癌的临床病理特征分类,三者的表达均与胃癌的病理组织学分级、临床分期及淋巴结转移有关(P<0.05),而与组织学类型无关(P>0.05)。三者在胃癌中的表达强度呈显著正相关(P<0.05)。H.pylori阳性的慢性萎缩性胃炎中PTTG、c-Myc及Ki67的表达均强于H.pylori阴性者。结论PTTG、c-Myc及Ki67参与了正常胃黏膜、胃癌前病变至胃癌的转化过程,三者在胃癌的发生发展中可能有协同作用,均可作为判定胃癌恶性程度,评估其侵袭性及转移的分子生物学指标。三者的表达可能与H.pylori感染有关。

Objectives To investigate the relationship of PTTG, c-Myc and Ki67 in gastric carcinoma, chronic atrophic gastritis and normal gastric mucosa, and study the potential pathogenesis of the gastric carcinoma. Methods Immunohistochemical staining （SP） was used to examine the expression of PTTG, c-Myc and Ki67 in 60 cases of gastric carcinoma, 20 cases of chronic atrophic gastritis and 10 cases of normal gastric mucosa. Results The positive rates of PTTG,c-Myc and Ki67 were all significantly different among normal gastric mucosa, chronic atrophic gastritis and gastric carcinoma. The expressions of them were related to histophathological grade, clinical stage and lymph node metastasis（P 〈0.05） ,but to histological type of gastric carcinoma（P 〉 0.05）. There was a positive correlation between the expression intensity of PTTG, c-Myc and Ki67 （ P 〈 0.05 ）. The expression of PTTG, c-Myc and Ki67 in chronic atrophic gastritis with H. pylori infection is higher than that without HP infection. Conclusions PTTG, c-Myc and Ki67 may be involved in the transformation from normal gastric mucosa to precancerous lesion with gastric carcinoma. They may play a cooperative role in the process of gastric carcinoma and may serve as a molecular biology index of evaluating infitration and metastasis. Expression of these proteins may be related to H. priori infection.