Pseudomonas sp.W2生长及对双酚A降解条件的研究

Conditions for Pseudomonas sp.W2 Culture and Bisphenol A Degradation

目的研究双酚A(BPA)降解菌(Pseudomonas sp.W2)的生长及对BPA的降解条件,为今后环境中BPA污染的实际治理提供实验依据。方法分别采用紫外扫描光谱分析和紫外光谱分析对Pseudomonas sp.W2菌株和BPA进行定量。分别绘制Pseudomonas sp.W2菌株的生长曲线及对BPA的降解曲线,并对其在不同的碳源、氮源、pH值、温度、通气情况下的生长情况及对BPA的降解情况进行分析。结果在一定条件下(25～35℃,pH值为6～8,250ml摇瓶中的培养基F装液量为50～100ml,有一定的碳源、氮源),Pseudomonas sp.W2的生长及对BPA降解作用较好。结论Pseudomonas sp.W2菌株的生长及对BPA的降解的条件较宽,这对其在实际中的应用具有一定意义。

Objective To study the condition for Pseudomonas sp. W2 culture and degradation of bisphenol A（BPA）. Methods The growth curve of Pseudomonas sp. W2 and BPA degradation curve were made concerned pH, temperature, aeration, carbon sources and nitrogen sources affecting Pseudomonas sp. W2 culture and BPA degradation. Results Under the conditions of pH=7, 25-35℃, certain carbon sources and nitrogen sources, Pseudomonas sp. W2 grew well and showed a good efficiency of BPA degradation. Conclusion Some wide conditions can meet the requirement of Pseudomonas sp. W2 growth and BPA degradation.