应用简化细胞分析软件进行角膜内皮细胞图像定量分析的可靠性研究

Assessment of the reliability of haman corneal endothelial morphologic parameters estimated using center by center method analysis

目的探讨应用简化细胞分析软件分析角膜内皮细胞图像时,抽样细胞数量对分析结果可靠性的影响。设计临床试验研究。研究对象同一志愿者右眼的中央角膜内皮细胞图像30张。方法用非接触角膜内皮镜拍摄清晰的内皮细胞图像。应用细胞中心分析法对内皮细胞图像进行定量分析,每次分析时抽取不同数量的细胞,从10个细胞至90个细胞,每一图像分析9次。比较不同抽样细胞数量所得各组分析结果间的差异。主要指标角膜内皮细胞密度、细胞密度的变异系数。结果各组内皮细胞密度的变异系数随着点数细胞数量的增加,呈现逐渐下降并趋于稳定的趋势,点数细胞数10、20、30、40、50至90的细胞密度变异系数分别为9.5、6.3、4.6、4.4、4.3至3.8。点数10个和20个细胞的两组内皮细胞密度与其他各组比较均有显著性差异(P<0.05);点数30至90个细胞的各组间比较均无显著性差异。结论应用细胞中心分析法对角膜内皮细胞图像进行定量分析时,点数细胞数量应大于等于30个。(眼科,2007,16:421-424)

Objective To evaluate the effect on reliability with different sample amount when human corneal endothelial morphologic parameters estimated using center by center method analysis. Design Clinical study. Participants Thirty central corneal endothelium photo-micrographs for the same eye.Methods Noncontact specular photomicrographs were obtained.Each photo-micrograph was analyzed with center by center method and was analyzed for 9 times by pointing different amount cells,from 10 to 90.Compare the diversity of comeal endothelial morphometric parameters between different groups.Main Outcome Measures Corneal endothclium density,coefficient of veriation.Results The coefficient of variation decreased and then kept to a same level when sample cell amount were added from 10 to 90. With the sample cell amounts varied from 10,20,30,40,50 to 90,the coefficient of variation was changed from 9.5,6.3,4.6,4.4,4.3 to 3.8.Corneal endothelium density had significant difference between the first two groups of 10 and 20 cell with the othe groups （P〈0.05）.There was no significant difference among the seven groups of 30 to 90 cells.Conclusion Using center by center method analysis need point out at leas 30 cells which could obtain reliable endothelial morphologic parameters.（Ophthalmol CHN, 2007, 16： 421-424）