脾虚证模型大鼠血液流变学及TXB_2、6-Keto-PGF_(1α)的变化

Changes of hemorheology and TXB_2 and 6-Keto-PGF_(1α) in blood of rats with syndrome of spleen-qi deficiency

目的观察脾虚证模型大鼠血流变学及血浆血栓素B2(TXB2)、6-酮-前列腺素F1α(6-Keto-PGF1α)及其比值的变化。方法采用游泳疲劳加饮食失节法复制大鼠脾虚证模型,测定大鼠造模3周、自然恢复1周时的血流变学和血浆TXB2、6-Keto-PGF1α及其比值。结果与对照组相比,模型组大鼠3周时150/s、38/s、10/s、5/s切变率下的全血黏度、还原黏度均显著升高(P<0.01或P<0.001或P<0.05),红细胞压积显著升高(P<0.01),聚集指数显著降低(P<0.001),变形指数无明显差异(P>0.05);血浆TXB2和6-Keto-PGF1α及TXB2/6-Keto-PGF1α均明显升高(P<0.05);模型组大鼠第4周时150 s-1、38 s-1、10 s-1、5 s-1切变率下的全血黏度、还原黏度仍显著升高(P<0.001);红细胞压积与变形指数无明显差异(P>0.05),聚集指数显著降低(P<0.001);血浆TXB2无明显差异(P>0.05),6-Keto-PGF1α显著升高(P<0.001),TXB2/6-Keto-PGF1α显著降低(P<0.05)。结论脾虚证大鼠存在血液高黏和易形成血栓状态,恢复期血液高黏同时伴有扩血管因素的增强。提示脾虚证有血流变学异常和血浆TXB2/6-Keto-PGF1α的平衡失调,主要涉及血小板和血浆因素的参与。

Objective To observe the changes of hemorheology and TXB2 ,6-Keto-PGF1α and their ratio in the blood of the rats with syndrome of spleen-qi deficiency. Methods The rat model with syndrome of spleen -qi deficiency was established by adopting excess fatigue caused by swimming and improper diet. the change of hemorheology and the contents of TXB2, 6-Keto-PGF1α and TXB2/6-Keto-PGF1α in plasma were determined in the 3rd and 4th week. Results Compared with the control group, in the 3rd week the whole blood viscosity and reduced viscosity under different shear increased significantly in the model group （P 〈 0.01 or P 〈 0. 001 or P 〈 0.05 ） ; hematocrit increased significantly（P 〈 0.01 ） ; the index of erythrocyte aggregation decreased significantly （ P 〈 0. 001 ） ; and the index of erythrocyte deformability did not change（P 〉 0.05 ）. In plasam of model group＇ s rats the content of TXB2, 6-Keto-PGF1α and TXB2/6-Keto-PGF1α were all increased obviously（P 〈0.05 ）. In the 4th week the whole blood viscosity and reduced viscosity under different shear still increased significantly（ P 〈 0.001 ） ; the hematocrit and the index of erythrocyte deformability did not change （P 〉 0.05 ） ; and the index of erythrocyte aggregationdecreased significantly （P 〈0.001 ） in the model group＇s rats. The content of TXB2 had no any change （P 〉0.05 ） but the content of 6-Keto-PGF1~ were higher than that in the control group （P 〈 0.001 ）. TXBJ6-Keto-PGF1= decreased markedly （P 〈 0.05）. Conclusion The rats with spleen-q/deficiency is in a state of high viscosity and easy to form thrombus. The one in convalescence is also with the dilatational factor of the blood vessel strengthened. It suggests that the hemorheology, TXB2 and 6-Keto- PGF1= of blood may exert abnormal in the syndrome of spleen-q/deficiency and the factors of platelet and plasma are involved mainly.