基于邻苯二胺聚合膜的纳米金自组装NADH修饰电极测定肾上腺素

A Novel Sensor of NADH Immobilized on Poly-o-phenylenediamine Film Self-Assembled Nano Gold and Its Application for the Determination of Epinephrine

研究了基于邻苯二胺聚合膜的纳米金自组装NADH修饰玻碳电极的制备，并探讨了肾上腺素在此修饰电极上的电化学行为，在pH=7．2的磷酸盐缓冲液中，肾上腺素在该修饰电极上产生一个灵敏的氧化峰，峰电流与肾上腺素浓度在3．0×10^-6～5．0×10^-5mol／L范围内呈良好的线性关系，检出限为3．15×10^-7mol／L．该方法可用于盐酸肾上腺素注射液中肾上腺素的检测．

The preparation of NADH-poly-o-phenylenediamine film-nano gold chemically modified glassy carbon electrode was studied, and the cyclic voltametric behavior of epinephrine （EP） on this modified electrode was investigated. EP showed a sensitive oxidation peak on the modified electrode in a phosphate buffer solution （PBS） of pH=7.2. The linear range of EP was 3. 0×10^-6-5. 0×10^-5 mol/L and the detection limit was 5.0 ×10^-7 mol/L. This method was applied to the measurement of EP in epinephrine hydrochloride injection samples with satisfactory results.