林州市贲门癌P53和PCNA与癌旁肠上皮化生的关系

P53 and proliferating cell nuclear antigen in patients with gastric cardiac adenocarcinoma with and without paracancerous intestinal metaplasia in Linzhou city

目的:探讨贲门腺癌(gastric cardia adeno- carcinoma,GCA)的P53和PCNA分子学特征与癌旁肠上皮化生(intestinal metaplasia,IM)的关系.方法:采用ABC免疫组织化学分析(immuno- histochemistry,IHC)法研究了70例癌旁含IM和不含IM的贲门癌P53和PCNA蛋白表达变化的特征.结果:癌旁含IM的GCA中P53蛋白阳性高表达率明显高于癌旁不含IM的GCA(33.33% vs 4.65%,P<0.01),而PCNA蛋白在两组中的阳性高表达率(74.07%和79.07%)比较无显著性差异(P>0.05).结论:癌旁含IM和不含IM的GCA形态相似,但可能具有不同的分子基础.

AIM： To characterize the expression of P53 and proliferating cell nuclear antigen （PCNA） proteins in gastric cardiac adenocarcinoma （GCA） with and without paracancerous intestinal metaplasia （IM）.METHODS： Immunohistochemical analysis （ABC method） was performed on tissues re- sected from 70 cases of GCA with and without paracancerous IM.RESULTS： The high positive expression rate for P53 protein in GCA tissues with paracancerous IM （33.33%） was significantly higher than that in GCA tissues without paracancerous IM （4.65%） （P 〈 0.01）, and the high positive expression rate for PCNA protein did not significantly differ be- tween the two groups （74.07% and 79.07%）.CONCLUSION： The high positive expression rate for P53 protein in GCA with paracancerous IM is significantly higher than without paracan- cerous IM, suggesting the possibility of a dif- ferent molecular basis in the two types of GCA with similar morphology.