Combination of allopurinol and hyperbaric oxygen therapy:A new treatment in experimental acute necrotizing pancreatitis?

瞄准：在试验性的老鼠在生物化学、组织病理学说的变化，氧化压力，和细菌的易位(BT ) 上调查 allopurinol 和高比重的氧(HBO ) 治疗的单个、联合的效果尖锐胰腺炎(AP ) 。方法：85 只 Sprague-Dawley 老鼠在学习被包括。85 只老鼠中的十五个被用作控制(假冒，组我) 。AP 在留下经由管内牛磺胆酸盐注入被导致七十只老鼠。幸存到尖锐引起坏死胰腺炎的正式就职的老鼠被使随机化进四个组。组 II 独自收到了 saline，组 III allopurinol，加 HBO 的组 IV allopurinol 和组 V HBO。浆液淀粉酶层次，氧化压力参数， BT 和组织病理学说的分数是坚定的。结果：浆液淀粉酶层次在与组 II 相比的组 III， IV 和 V 是更低的(974 +/- 110， 384 +/- 40， 851 +/- 56，和 1664 +/- 234 U/L，分别地 P 【 0.05，为所有) 。联合揭示的二种处理选择显著地降低 median [25-75 百分位数] 组织病理学说的分数什么时候与单个政府相比(13 [12.5-15 ] 在 allopurinol 组， 9.5 [7-11.75 ] 在 HBO 组，并且 6 [4.5-7.5 ] 在联合的组， P 【 0.01 ) 。氧化压力标记在与控制相比的所有处理组显著地更好。进胰和 mes 伤寒淋巴结的细菌的易位在与组 II 相比的组 III ， IV 和 V 是更低的(54%，23%，50%对为到胰的易位的100%，并且62%，46%，58%对为到 mes 伤寒淋巴节点的易位的100%，分别地 P 【 0.05 为所有)。结论：当在试验性的老鼠 AP 独立管理了时，现在的学习证实 HBO 和 allopurinol 处理的利益。这些处理选择的联合看起来更有效地阻止胰腺的损害参数的前进。

AIM： To investigate the individual and combined effects of allopurinol and hyperbaric oxygen （HBO） therapy on biochemical and histopathological changes, oxidative stress, and bacterial translocation （BT） in the experimental rat acute pancreatitis （AP）. METHODS： Eighty-five Sprague-Dawley rats were included in the study. Fifteen of the eighty-five rats were used as controls （sham, Group I ）. AP was induced via intraductal taurocholate infusion in the remaining seventy rats. Rats that survived to induction of acute necrotizing pancreatitis were randomized into four groups. Group H received saline, Group m allopurinol, Group IV allopurinol plus HBO and Group v HBO alone. Serum amylase levels, oxidative stress parameters, BT and histopathologic scores were determined. RESULTS： Serum amylase levels were lower in Groups Ⅲ, Ⅳ and v compared to Group H （974 ± 110, 384 ± 40, 851 ＋ 56, and 1664 Ⅳ 234 U/L, respectively, P 〈 0.05, for all）. Combining the two treatment optionsrevealed significantly lower median [25-75 percentiles] histopathological scores when compared to individual administrations （13 [12.5-15] in allopurinol group, 9.5 [7-11.75] in HBO group, and 6 [4.5-7.5] in combined group, P 〈 0.01）. Oxidative stress markers were significantly better in all treatment groups compared to the controls. Bacterial translocation into the pancreas and mesenteric lymph nodes was lower in Groups m, iV and v compared to Group H （54%, 23%, 50% vs 100% for translocation to pancreas, and 62%, 46%, 58% vs 100% for translocation to mesenteric lymph nodes, respectively, P 〈 0.05 for all）. CONCLUSION： The present study confirms the benefit of HBO and allopurinol treatment when administered separately in experimental rat AP. Combination of these treatment options appears to prevent progression of pancreatic injury parameters more effectively.