管花肉苁蓉松果菊苷次生代谢部位研究

Study on secondary metabolic organ of echinacoside in herbs of Cistanche tubulosa

目的:通过对管花肉苁蓉寄生生长和柽柳-管花肉苁蓉不同部位松果菊苷含量的研究,为明确管花肉苁蓉松果菊苷的次生代谢部位及调控提供理论依据。方法:高效液相系统分析了指标性成分松果菊苷含量,比较分析了管花肉苁蓉干物质积累量、松果菊苷积累量和寄生柽柳根粗的相关关系。结果:随着管花肉苁蓉干物质积累量的增加,植株内的松果菊苷积累量显著增加,两者与寄生的柽柳根粗呈极显著正相关关系。吸器韧皮部的松果菊苷质量分数为15.53%,高于吸器木质部、管花肉苁蓉植株及其他部位的松果菊苷含量。结论:吸器韧皮部可能是管花肉苁蓉松果菊苷的次生代谢部位。

Objective： It could give some theory support of confirming the secondary metabolism organ and regulation of echinacoside in Cistanche tubulosa by searching parasitic growth of C. tubulosa and echinacoside variation in different organs of host and parasite. Method： The echinacoside content was analyzed by HPLC. The relationship between dry matter accumulation and echinaco- side accumulation of C. tubulosa as the wen as root diameter of host were comparatively analyzed. Result. With the increase of dry matter accumulation of C. tubulosa, echinacoside accumulation increased significantly, and both of them were in significantly positive correlated with the root diameter of host. Echinacoside content in haustorium phloem was 15.53%, higher than that of haustorium xylem, C. tubulosa plant and other organs. Conclusion： Haustorium phloem was probably the secondary metabolism organ of echinacoside in C. tubulosa.