越鞠丸提取物YJ-XCC1Z3抗抑郁作用研究

Experimental studies on treatment of depression with YJ-XCC1Z3 in mouse models

目的:观察越鞠丸提取物YJ-XCC1Z3用于抑郁症的实验治疗效果。方法:YJ-XCC1Z3分高、低剂量405,135 mg.kg-1分别给予小鼠灌胃。采用自主活动测定仪,观测YJ-XCC1Z3对小鼠自主活动的影响;采用2种行为绝望模型小鼠悬尾试验和小鼠强迫游泳试验,观察YJ-XCC1Z3对小鼠不动时间的影响;采用利血平拮抗实验,观测YJ-XCC1Z3对小鼠体温及脑内单胺类神经递质代谢的作用;采用5-羟色胺酸(5-HTP)诱导小鼠甩头增强试验,观测YJ-XCC1Z3对小鼠甩头次数的影响。结果:YJ-XCC1Z3组小鼠的自主活动与对照组比较没有显著差异;YJ-XCC1Z3 405 mg.kg-1能明显缩短小鼠强迫游泳不动时间与小鼠悬尾不动时间;YJ-XCC1Z3 405,135 mg.kg-1均可显著拮抗利血平所致的小鼠体温下降;YJ-XCC1Z3 405 mg.kg-1组可明显增加5-HTP诱导小鼠甩头总次数;YJ-XCC1Z3 405,135 mg.kg-1均使5-羟色胺(5-HT)、去甲肾上腺素(NE)的含量增加,YJ-XCC1Z3 405 mg.kg-1组可使多巴胺含量减少;YJ-XCC1Z3 135 mg.kg-1使5-羟基吲哚乙酸(5-HIAA)含量增加,对高香草酸(HVA)和3,4-二羟基苯乙酸(DOPAC)的含量无影响;YJ-XCC1Z3 405,135 mg.kg-1均使5-HIAA/5-HT比值减少。结论:YJ-XCC1Z3可以改善小鼠抑郁状态的行为,具有一定的抗抑郁作用,并无精神运动兴奋作用。YJ-XCC1Z3可通过增加脑内5-HT,NE的含量而发挥抗抑郁作用,其中对5-HT的影响与YJ-XCC1Z3降低脑内5-HT的代谢有关。

Objective： To evaluate the pre-clinical effect of YJ-XCC1Z3 on the treatment of depression with the mice mouse. Method： YJ-XCC1Z3 was administered at the dose of 405 mg · kg^-1 and 135 mg · kg^-1 to observe the locomotor activity with the mouse locomotor activity recorder apparatus, to observe the effect of YJ-XCC1Z3 on the duration of immobility in the mouse forced swimming test and tail suspension test, to observe the effect of YJ-XCC1Z3 on the body temperature and the metabolism of monoamine in mouse brain in the mouse model of reserpine induced hypothermia, and to observe the effect of YJ-XCC1Z3 on the times of 5-HTP induced head-twitches in mice. Result： There were no significant changes in the locomotor activity, but a significant reduction in the immobility time was observed in the mice treated with YJ-XCC1Z3 405 mg · kg^-1 and imipramine in the forced swimming test and the tail suspension test. YJ-XCC1Z3 135 mg · kg^-1and 405 mg · kg^-1 could improve the range of reserpine induced hypothermia in mice, and the latter could also enhance the times of 5-HTP induced head-twitches in mice. YJ-XCC1Z3 405 mg · kg^-1 and 135 mg · kg^-1 could increase the content of 5-HT and NE and decrease the ratio of 5-HIAA/5-HT in mouse brain, but the dose of 405 mg · kg^-1 could decrease the content of DA. The dose of 405 mg · kg^-1could increase the content of 5-HIAA and had no obvious effect on the content of HVA and DOPAC. Conclusion： YJ-XCC1Z3 shows potent antidepressant effect by improving the behaviour of the mouse in depression and not inducing hyperlocomotion in the mice. This effect results in the increase of the content of 5-HT and NE in the mouse brain. YJ-XCC1Z3 can decrease the metabolism of 5-HT to effect the content of 5-HT.