三七皂苷对阿霉素致心肌损伤保护作用的研究

Study on protective effects of Panax notoginseng saponins on doxorubicin-induced myocardial damage

目的：研究三七皂苷对阿霉素所致心肌损伤的保护作用及其对阿霉素抗肿瘤活性的影响。方法：15mg·kg^-1腹腔注射阿霉素造成小鼠急性心肌损伤模型，观察不同剂量三七皂苷（25，50，100mg·kg^-1）干预对动物血清心肌酶谱指标及心肌组织中抗氧化酶活力的影响；采用大鼠心肌细胞H9C：，考察不同质量浓度三七皂苷（6．25～100mg·kg^-1）减轻阿霉素心肌细胞毒性的作用；以MTF法测定三七皂苷对阿霉素抑制肿瘤细胞生长作用的影响。结果：阿霉素显著升高小鼠血清心肌酶指标，降低心肌组织抗氧化酶活力，不同剂量三七皂苷均能减轻心肌损伤、抑制心肌组织抗氧化酶活力的下降。阿霉素抑制离体培养心肌细胞的活力，三七皂苷能提高细胞存活率，但并不拮抗阿霉素对肿瘤细胞生长的抑制作用。结论：三七皂苷在体内和体外均显示减轻阿霉素心脏毒性的作用，同时并不影响后者抑制肿瘤细胞生长的作用。

Objective： To study the effects of Panax notoginseng saponins （PNS） on acute doxorubicin-induced myocardial injury in mice and the anti-tumor efficiency of doxorubicin. Method： Mice were given a dose of 15 mg·kg^-1 doxorubicin ip alone or in combination with 25, 50, 100 mg·kg^-1 PNS ig, 5 days before doxorubicin administration and following 3 days. Cardiotoxic effects were measured by serum levels of dehydragenase （LDH）, creatine kinase （CK） and creatine kinase isoenzyme （CK-MB） and activities of antioxidant enzymes in heart tissue. In vitro experiments were performed using embryonic rat heart cell H9 C2 to assess the protec- tive effect of PNS （6. 25 - 100 mg·kg^-1 ） against doxorubicin on cell viability. Anti-tumor efficiency of doxorubicin was evaluated by cytotoxic experiments using three cancer cell lines. Result： Pretreatment with PNS significantly lowered the levels of serum LDH, CK and CK-MB, and normalized myocardial superoxide dismutase, glutathione peroxidase and catalase activities. PNS also attenuated the inhibitory effect of doxorubicin on the viability of H9 C2 cells, but did not compromise its inhibitory effect on proliferation of cancer cells. Condusion： PNS was demonstrated to attenuate doxorubicin-induced myocardial damage without compromising its anti-tumor activity.